比较不同温度对乳腺癌侵袭转移能力的影响

目的 比较研究不同温度的热疗对人乳腺癌株MCF-7细胞侵袭转移能力的影响及其相关分子机制.方法 水浴热疗43、45和47℃ 3组MCF-7细胞各30 min,37℃为对照组.采用免疫组织化学染色法检测MCF-7细胞转化生长因子.β1(TGF-β1)、血管内皮生长因子(VEGF)蛋白的表达;明胶酶谱法检测细胞上清液中基质金属蛋白酶(MMP)-2、MMP-9的分泌及活性.结果 热疗对MMP-2、MMP-9的释放和激活均有明显的抑制作用(P<0.05);且对MMP-2活性的抑制作用有明显的温度依赖性,随加热温度增加,抑制作用越明显(P<0.05).各热疗组TGF-β1和VEGF的表达均较对照组显著下降(P<0.05),但不同温度热疗组之间差异无统计学意义(P>0.05);结论 43、45、47 β热疗均可下调MCF-7细胞中TGF-β1、VEGF的蛋白表达及抑制MMP.2、MMP-9的分泌和活性,从而抑制人乳腺癌细胞侵袭转移能力.

Comparison of hypothermia effects with different temperatures on human breast adenocarcinoma invasiveness and metastasis

Objective To study the effects of hyperthermia with different temperature on inhibiting in-vasiveness and metastasis of human breast adenocarcinoma cells (MCF-7)and its possible mechanism. Methods MCF-7 was treated by heating with different temperatures (43 ℃,45℃ and 47℃)for 30 minites and 37 ℃ as control. Expression of matrix metalloproteinase( MMP)-2,MMP-9, vascular endothelial growth factor(VEGF) ,transforming growthfactor(TGF)-β1 of MCF-7 after different heating temperatures treatment was examined by immuno-cytochemical technique;The effect of hyperthermia on MMP-2,MMP-9 expression,activity were also studied by gelatin zymographic assays. Results Comparing with 37 ℃ group,hyperthermia with 43℃ ,45℃ and 47 ℃ also decreased MMP-2,MMP-9 enzymatic activitiesl which is temperature-dependant( P <0.05). The VEGF,TGF-β1 protein expression in 43 ℃ ,45 ℃ and 47 ℃ groups decreased significantly( P <0.05), but there is no differeme between heating groups ( P > 0.05 ). Conclusion Hyperthermia as potential treatment inhibits invasion by a process involved in multiple regulatory molecules in MCF-7.