| 链霉素抗性突变理性筛选avermectin高产菌株 |
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| 引用本文： | 陶纯长,谌颉,郭美锦,郭元昕,储炬,庄英萍,张嗣良.链霉素抗性突变理性筛选avermectin高产菌株[J].中国抗生素杂志,2002,27(9):521-523,528. |
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| 作者姓名： | 陶纯长 谌颉 郭美锦 郭元昕 储炬 庄英萍 张嗣良 |
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| 作者单位： | 华东理工大学生物反应器工程国家重点实验室,上海,200237 |
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| 摘 要： | 为提高菌株avermectin的产量，本文以链霉素抗性为选择压力，对除虫链霉菌Streptomyces avermi-tilis进行紫外诱变（253.7nm,30w，照射时间45s)，得到在摇瓶发酵水平比出发菌株产量提高25%以上的4株突变株，其中2株提高水平达30%以上，实验表明了链霉素抗性突变与产抗生素突变之间的密切联系。同时，对其机理和意义作了一些探讨。
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| 关 键 词： | Avermectin 除虫链霉菌 链霉素抗性 理性筛选 |
| 文章编号： | 1001-8689(2002)09-0521-03 |
Streptomycin-resistant rational screening for high yield avermectins producing strain |
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| Tao Chun chang,Chen Jie,Guo Mei jin,Guo Yuan xin,Chu Ju,Zhuang Ying ping and Zhang Si liang.Streptomycin-resistant rational screening for high yield avermectins producing strain[J].Chinese Journal of Antibiotics,2002,27(9):521-523,528. |
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| Authors: | Tao Chun chang Chen Jie Guo Mei jin Guo Yuan xin Chu Ju Zhuang Ying ping and Zhang Si liang |
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| Abstract: | To screen high avermectin producing strain,streptomycin resistant Strptomyces avermitilis was treated with ultraviolet (253 7nm, 30w, 45s). Four mutants were obtained, their productivities increased by more than 25% including 2 mutants by more than 30%, Results showed that there were close relationships of mutations between streptomycin resistance and antibiotic productivity, and the mechanism was also discussed. |
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| Keywords: | Avermectins Streptomyces avermitilis Streptomycin resistance Rational screening |
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