组织工程化肌腱修复喙锁韧带术后的短串联重复位点检测

目的 用短串联重复位点检测方法，评价组织化肌妥临床应用修复喙锁韧带损伤的效果。方法 １９９９年９月采用人胚肌腱细胞与材料体外复合增减鸺建组织工程化肌腱，修复喙锁韧带损伤。术后６个月，行内固定取出术时，钳取微量组织作组织学观察，并提取ＤＮＡ，并提取ＤＮＡ，检测Ｄ２Ｓ１７５４，及Ｃｙａｒ０４基因座。结果 患者术后功能恢复良好，无局部及全身免疫排斥反应，Ｄ２Ｓ１７５１及Ｃｙａｒ０４基因座电泳分型中含有非

SHORT TANDEM REPEAT LOCI EXAMINATION AFTER REPAIR OF CORACOCLAVICULAR LIGAMENT INJURY BY TISSUE ENGINEERED TENDON

Objective To evaluate the result of clinical application in the repair of coracoclavicular ligament injury by tissue engineered tendon using the technique of short tandem repeat loci examination.Methods In september 1999, human embryonic tendon cells and artificial materials were co cultured in vitro to construct tissue engineered tendon, which repaired coracoclavicular ligament injury. After 6 months of operation, micro tissue were sampled during the operation of removal of internal fixation, and morphological characteristics were examined by HE staining, DNA of tissues were extracted to examine D3S1754 and Cyar04 gene loci. Results The shoulder function of the patient was recovered well after operation, and no local or systemic immunological rejection were occurred. The electrophoresis typing showed 13/14 at D3S1754 and 8/9 at Cyar04 in the tissue of tissue engineered tendon, while the autogenous ligament were 13/13 and 8/8 at D3S1754 and Cyar04 loci respectively, which suggested that the tissue engineered tendon was survived in vivo . Conclusion The examination of short tandem repeat loci is a better index to evaluate the survival situation of tissue engineered tissue after transplantation in clinical application.