LKB1基因对乳腺癌细胞促进微血管生成的因子基因表达的影响

目的 研究LKB1基因对乳腺癌细胞促进微血管生成的因子基因表达的影响及机理。方法筛选LKB1基因转染的MDA-MB-435乳腺癌细胞，随机取样一株高表达细胞株和一株低表达细胞株，同时与未转染细胞株和空质粒转染细胞株比较。用RT-PCR观察促进微血管生成的因子的基因表达和分泌情况，并用Western blot对其蛋白定量分析。并采用Tramswell试验(Matrigel胶侵袭力检测法)对其进行膜穿透能力检测。结果 无论从mRNA还是蛋白水平上分析LKB1转染的乳癌细胞的促进微血管生成的因子基因表达VEGF和bFGF较未转染细胞和空质粒细胞为低，且高表达LKBl的细胞较低表达者为低。相似的结果在Tramswell试验得到证实。结论 LKB1基因作为一种抑癌基因在乳腺癌细胞的侵袭和转移中起到重要作用，特别对促进微血管生成的因子作用不容忽视。

The study of LKB1 gene on vascular endothelial growth factor by breast cancer cells

Objective To investigate the effect of LKB1 gene on vascular endothelial growth factor by breast cancer cells,and the underlying mechanisms. Methods To screen out the corresponding positive clone of MDA-MB-435/LKB1 ,draw out in random of MDA-MB-435/LKB1(H)and MDA-MB-435/LKB1(L).To contrast with MDA-MB-435 and MDA-MB-435/vec.VEGF and bFGF mRNA contents were assayed by RT-PCR. Protein contents were determined by Western Blot analysis. The alteration of cellular migration property was evaluated by cell migration through a Matrigel model basement membrane. Results In MDA-MB-435/LKB1 ,the expression of VEGF and bFGF was detected by RT-PCR , Western blot were reduced,too. Moreover,the same result in cell migration ability through a Matrigel model basement membrane. Conclusion Our results show the invasion and metastasis associated genes were altered in MDA-MB-435 with LKB1.Especially the effect of the vascular endothelial growth factor.