艾灸“足三里”对胃黏膜损伤大鼠内源性保护因子含量及相关蛋白表达的影响

目的观察艾灸"足三里"穴对孤束核损毁术后胃黏膜损伤大鼠内源性保护因子含量及相关蛋白的表达,探讨孤束核在艾灸保护胃黏膜损伤神经通路中的作用。方法按随机数字表法将48只健康SPF级SD大鼠分为4组:正常对照组,模型组,艾灸+模型组(艾灸组),艾灸+模型+孤束核损毁组(艾灸加孤束核损毁组),每组各12只。其中艾灸加孤束核损毁组予双侧孤束核损毁,护理3 d后,模型组和艾灸组大鼠均以无水乙醇(6 m L/kg)灌胃造模,并用阿司匹林混悬液(200 mg/kg)连续3 d灌胃以维持胃黏膜损伤,正常对照组用等量生理盐水灌胃,第4天进行艾灸"足三里"处理(正常对照组和模型组只绑不灸),每日2次,连续3 d。取血清及胃组织,计算胃黏膜损伤指数(UI),用ELISA法检测胃黏膜组织中表皮生长因子(EGF)、一氧化氮(NO)含量,用Western-blot法检测胃黏膜组织中抗凋亡蛋白(Bcl-2)、凋亡蛋白(Bax)的蛋白表达情况。结果与正常对照组比较,模型组胃黏膜损伤明显(P0.05);艾灸组UI较模型组下降(P0.05)。与模型组比,艾灸组血清、胃组织EGF、NO含量明显升高(均P0.05);艾灸加孤束核损毁组血清、胃组织EGF、NO含量较艾灸组低(均P0.05)。艾灸组和艾灸加孤束核损毁组Bcl-2蛋白表达较模型组有明显上升,而Bax表达明显下降(P0.05),细胞凋亡指数(AI)比值上升(P0.05)。结论艾灸足三里穴可调节机体内源性保护因子及蛋白的表达,保护胃黏膜,并受孤束核损毁的影响。证明孤束核是艾灸足三里穴产生胃黏膜保护效应信号传导通路中的重要调节部位。

Effects of moxibustion at Zusanli(ST36) on endogenous protective factors and related proteins expression in gastric mucosal lesions rats after dama-ging nucleus tractus solitarii

Objective To study the role of nucleus tractus solitarii ( NTS ) on the protective effects of moxibustion on gastric mucosa lesion in the nervous pathway by investigating the effects of moxibustion at"Zusanli" ( ST 36 ) on endogenous protective factors and gap-associated protein expression of gastric mu-cosal lesion rats after damaging their nucleus tractus solitarii ( NTS) . Methods 48 healthy SD rats, SPF grade, were divided into four groups using random number table ( each n=12 ): normal group, model group, moxibustion group, moxibustion + NTS damaged group. Three days after bilateral NTS damaged in moxibustion + NTS damaged group, gastric mucosal lesion rat model was induced by intragastrical ( i. g. ) administration of anhydrous ethanol at dose of 6 mL/kg and aspirin mixed suspension at dose of 200 mg/kg for consecutive three days. The next day, rats in moxibustion group and moxibustion + NTS damaged group were treated by moxibustion at Zusanli ( ST 36 ) twice daily for consecutive three days. All rats were sacrificed, then their serum and gastric tissue were collected. Ulcer index ( UI) was calcu-lated, and the expressions of epidermal growth factor ( EGF) , nitric oxide ( NO) , Bcl-2 anti-apoptotic proteins ( Bcl-2 ) and pro-apoptotic protein ( Bax ) were detected. Results Compared with normal group, UI in model group increased significantly(P <0. 05), decreased in moxibustion group(P <0. 05). Compared with model group, content of EGF and NO in serum and gastric tissue increased remark-ably in moxibustion group(P<0. 05)which were higher than those in moxibustion + NTS damaged group ( P<0 . 05 ) . Protein expression of Bcl-2 increased significantly in moxibustion group and moxibustion +NTS damage group compared with model group, while expression of Bax protein decreased with increasing of AI ( P<0 . 05 ) . Conclusion Treatment of moxibustion at Zusanli ( ST 36 ) can regulate the expres-sion of endogenous protective factors and proteins to protect rats’ gastric mucosa, which was affected by damaging NTS. It is suggested that NTS be the important regulatory location in the signaling pathway of protective effects of moxibustion at Zusanli on gastric mucosal.