| TNP-470对肺癌细胞诱导的血管内皮细胞增殖的抑制作用 |
| |
| 引用本文： | 吴晓娟,杨向红. TNP-470对肺癌细胞诱导的血管内皮细胞增殖的抑制作用[J]. 中国医科大学学报, 2004, 33(5): 405-407 |
| |
| 作者姓名： | 吴晓娟 杨向红 |
| |
| 作者单位： | 中国医科大学基础医学院实验病理研究室,辽宁,沈阳,110001 |
| |
| 摘 要： | 目的:应用体外细胞培养法探讨O-(氯乙酰-氨甲酰基)烟曲霉素(TNP-470)对肺癌细胞诱导的血管内皮细胞增殖的影响及其可能机制.方法:免疫细胞化学方法检测血管内皮细胞增殖细胞核抗原(PCNA)、胚胎肝激酶1(Flk-1)、纤维细胞生长因子受体(FGFR)的表达.结果:TNP-470以剂量依赖方式抑制肺癌细胞条件培养液或细胞粉碎液诱导的血管内皮细胞PCNA,Flk-1,FGFR的表达,与对照组比较差异均显著(P<0.05).结论:TNP-470能够相对特异地抑制血管内皮细胞增殖,可能与通过降低内皮细胞表面Flk-1,FGFR的表达,进而减弱酪氨酸激酶受体介导的细胞增殖途径有关.
|
| 关 键 词： | O-(氯乙酰-氨甲酰基)烟曲霉素 人脐静脉内皮细胞 肺癌细胞 |
| 文章编号： | 0258-4646(2004)05-0405-03 |
| 修稿时间： | 2003-06-04 |
Inhibition of TNP-470 on proliferation of vascular endothelial cells induced by pulmonary cancer cells |
| |
| WU Xiao-juan,YANG Xiang-hong. Inhibition of TNP-470 on proliferation of vascular endothelial cells induced by pulmonary cancer cells[J]. Journal of China Medical University, 2004, 33(5): 405-407 |
| |
| Authors: | WU Xiao-juan YANG Xiang-hong |
| |
| Abstract: | Objective: To discuss the influence and poss ible mechanism of TNP-470 on the cultured cell in vitro. Methods: The expressions of proliferating cell nuclear gene (PCNA), fetal liver kina se 1 (Flk-1), fibroblast growth factor receptor (FGFR) of endothelial cells w ere tested by using immunocytochemical analysis, positive cell-count, and image analysis. Results: The TNP-470 inhibited the expressions of P CNA, Flk-1, and FGFR of human umbilical vein endothelial cell induced by pulmon ary cancer cell dose-dependently compared with conditional group (P<0.05). Conclusion: The TNP-470 reduced the proliferation of endothe lial cells mediated by receptor tyrosine kinases(RTKs) pathway by weakening the expressions of Flk-1 and FGFR of endothelial cells. |
| |
| Keywords: | TNP-470 human umbilical vein endothelial cell pulmonary cancer cell |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
