| 豆豉纤溶酶RGDS-载酶脂质体的体内溶栓效果和靶向性评价 |
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| 引用本文： | 王成涛,籍保平,曹雁平,孙宝国,刘旭东. 豆豉纤溶酶RGDS-载酶脂质体的体内溶栓效果和靶向性评价[J]. 生物医学工程学杂志, 2010, 0(2) |
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| 作者姓名： | 王成涛 籍保平 曹雁平 孙宝国 刘旭东 |
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| 作者单位： | 北京工商大学化学与环境工程学院;中国农业大学食品与营养工程学院;莱州出入境检验检疫局; |
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| 基金项目： | 国家重点基础研究发展计划项目资助(973项目,2007CB707802); 北京市属高校“中青年骨干人才培养计划”项目资助(0142131301) |
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| 摘 要： | 研究评价了豆豉纤溶酶(Subtilisin FS33)RGDS-载酶脂质纳米粒的溶栓效果和靶向性,探讨RGDS-脂质体作为载体导向于血栓灶的可能性。不同剂量荧光标记RGDS-载酶脂质体给予血栓模型动物,考察其对机体抗凝血和纤溶功能的影响,以及组织器官的荧光素分布,结果发现,Subtilisin FS33高剂量组注射15 min～2 h,机体的APTT、PT和TT都明显延长(P<0.05,P<0.01),纤溶酶活力明显增高(P<0.05),血浆FDP的含量升高(P<0.05,P<0.01),D-di mer均呈阳性,ELT值降低;低剂量组也有相似趋势(P<0.05);RGDS-载酶脂质体组的大鼠组织切片中脑静脉和肾小球毛细血管中无明显血栓,只有少量红细胞聚积,并且在脑、心脏和肝组织中荧光物的含量显著高于无RGDS-载血清白蛋白脂质体组(空白对照组)。试验表明静注RGDS-载酶脂质体可明显增强机体的抗凝和纤溶能力,RGDS-载酶脂质体具有一定的血栓靶向性。
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| 关 键 词： | 豆豉纤溶酶 血栓模型 RGDS-载酶脂质体 纳米粒 溶栓 靶向性 |
Evaluating Thrombolytic Efficacy and Thrombus Targetability of RGDS-liposomes Encapsulating Subtilisin FS33 in Vivo |
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| Wang Chengtao, Ji Baoping Cao Yanping Sun Baoguo Liu Xudong. Evaluating Thrombolytic Efficacy and Thrombus Targetability of RGDS-liposomes Encapsulating Subtilisin FS33 in Vivo[J]. Journal of biomedical engineering, 2010, 0(2) |
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| Authors: | Wang Chengtao Ji Baoping Cao Yanping Sun Baoguo Liu Xudong |
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| Affiliation: | Wang Chengtao1,2 Ji Baoping2 Cao Yanping1 Sun Baoguo1 Liu Xudong3 1(College of Chemical , Environmental Engineering,Beijing Technology & Business University,Beijing 100048,China)2(College of Food Science , Nutritional Engineering,China Agricultural University,Beijing 100083,China) 3(Laizhou Entry-exit Inspection , Quarantine Bureau,Laizhou 261400,China) |
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| Abstract: | A novel fibrinolytic enzyme subtilisin FS33,which exhibits much higher activity for decomposing fibrin than urokinase,was purified from Douchi,a traditional soybean-fermented food in China.In order to increase bio-utilization and thrombus targetability of subtilisin FS33 labeled by fluorescein isothiocyanate(FITC),the surface modified liposomes encapsulating subtilisin FS33 and FITC with a synthetic peptide Arg-Gly-Asp-Ser(RGDS),being putatively a specific antagonist of fibrinogen receptor on platelet membr... |
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| Keywords: | Subtilisin FS33 Thrombi model RGDS-liposomes Nanoparticles Thrombolytic Targetability |
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