Electron microscopy of pea enation mosaic virus in plants and aphid vectors |
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| Authors: | E Shikata K Maramorosch R R Granados |
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| Affiliation: | 1. Cesar Australia, Brunswick, VIC 3056, Australia;2. Pest and Environmental Adaptation Research Group, School of Biosciences, Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Parkville, VIC 3052, Australia;3. Section for Bioscience and Engineering, Department of Chemistry and Bioscience, Aalborg University, Aalborg 9220, Denmark;4. School of Agriculture, Food and Ecosystem Sciences, University of Melbourne, Parkville, VIC 3052, Australia;1. Research Center for Immunodeficiencies, Children''s Medical Center, Tehran University of Medical Sciences, Tehran, Iran;2. Network of Immunity in Infection, Malignancy and Autoimmunity (NIIMA), Universal Scientific Education and Research Network (USERN), Tehran, Iran;3. Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran;1. Department of Biology, University of Rochester, Rochester, NY 14610, USA;2. Current address: Department of Computational Biology, Indraprastha Institute of Information Technology Delhi; New Delhi, India;3. School of Biological Sciences, University of Nebraska-Lincoln |
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| Abstract: | Ultrathin sections were prepared from tissues of pea plants infected with pea enation mosaic virus and from pea aphid vectors of this virus. Infected plant tissues and various organs of the insect vector were excised, fixed with glutaraldehyde-osmic acid, embedded in epoxy resin, sectioned, stained with uranyl acetate and lead, and examined with a Siemens Elmiskop I in an attempt to locate the position of virions in cells. Numerous spherical particles, about 28 mμ in diameter, were found in the cytoplasm and central vacuoles of necrotic and nonnecrotic pea leaf and pod enations, but not in the mitochondria. Similar particles were detected in the fat body and gut lumen of viruliferous pea aphids. Dip preparations of sap from diseased plants also contained particles of the same size and shape. No similar particles were found in healthy pea tissues, in virus-free pea aphids, or in dip preparations from healthy plant material. The spherical particles probably represent the virions of pea enation mosaic virus. Unusual formations of particles were observed in vacuolated structures of plant cells. In rare instances virus microcrystals were found in the cytoplasm of plant cells. Pea enation mosaic virus is the first circulative aphid-borne virus localized by electron microscopy in plants and insects in situ. |
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