| 用PCR检测心肌疾病患儿心肌组织EV－RNA |
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| 引用本文： | 王雪梅,叶鸿瑁,秦宝贵,韩玲,刘晓晔. 用PCR检测心肌疾病患儿心肌组织EV－RNA[J]. 北京大学学报(医学版), 1996, 0(3) |
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| 作者姓名： | 王雪梅 叶鸿瑁 秦宝贵 韩玲 刘晓晔 |
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| 作者单位： | 北京医科大学第三医院儿科，北京医科大学第三医院武警总医院儿科，北京医科大学第三医院安贞医院 |
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| 摘 要： | 目的：研究肠道病毒感染与心肌炎和心肌病的关系。方法：用聚合酶链反应及核酸杂交技术检测心肌疾病病人心肌组织中及血清肠道病毒ＲＮＡ，同时用酶联免疫吸附试验测血清柯萨奇Ｂ病毒特异ＩｇＭ、ＩｇＧ抗体，取非心肌炎者心肌、血清作为对照。结果：有心肌损伤病人可用ＰＣＲ技术证明其心肌或血清中存在病毒ＲＮＡ，而无心肌损伤病人及对照组均阴性。结论：应用ＰＣＲ技术配合免疫学检测对于研究病毒感染与心肌炎和心肌病病因的关系很有帮助。
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| 关 键 词： | 心肌炎／病毒学，心肌／病毒学，肠病毒／分离和提纯，聚合酶链反应 |
DETECTION OF ENTEROVIRUS RNA IN MYOCARDIAL TISSUE FROM PATIENTS WITH MYOCARDITIS AND CARDIOMYOPATHY BY POLYMERASE CHAIN REACTION |
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| WANG Xuemei, YE Hongmao, QIN Baogui, HAN Ling, LIU Xiaoye. DETECTION OF ENTEROVIRUS RNA IN MYOCARDIAL TISSUE FROM PATIENTS WITH MYOCARDITIS AND CARDIOMYOPATHY BY POLYMERASE CHAIN REACTION[J]. Journal of Peking University. Health sciences, 1996, 0(3) |
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| Authors: | WANG Xuemei YE Hongmao QIN Baogui HAN Ling LIU Xiaoye |
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| Abstract: | Objective:To study the relationship between enterovirus(EV)infection and myocarditis and cardiomyopathy.Methods:Polyrnerase chain reaction(PCR)and Northern Blotting were used to detect EV-RNA in myocardial tissue and sera from patients with myocardial disease. Sera of these patients were also detected for coxsackievirus B(CVB)specific IgM and IgG antibodies by enzyme-linked-im-munosorbent assay.Cases with no cardial disease were detected as controls. Results:Cases with my-cardial damage were positive for EV-RNA in myocardial tissue,The controls were negative,Couclu-sion:PCR with immunoassay is very helpful for studying the relationship between EV infection and my-ocarditis and cardiomyopathy. |
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| Keywords: | Headings Myocarditis/viru Myocardium/viru Enteroviruses/isol Polymerase chain re-action |
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