| 临床膀胱癌组织中miRNA的差异表达研究 |
| |
| 引用本文： | 夏伟,宋涛,李洁,黄宝春,李少华,丁红梅,王天有,邵宁生. 临床膀胱癌组织中miRNA的差异表达研究[J]. 军事医学科学院院刊, 2010, 34(6): 540-542 |
| |
| 作者姓名： | 夏伟 宋涛 李洁 黄宝春 李少华 丁红梅 王天有 邵宁生 |
| |
| 作者单位： | [1]军事医学科学院基础医学研究所,北京100850 [2]解放军总医院泌尿外科,北京100853 [3]首都儿科研究所附属儿童医院,北京100020 |
| |
| 基金项目： | 国家重点基础研究发展计划(973计划)，国家自然科学基金，国家高技术研究发展计划(863计划) |
| |
| 摘 要： | 目的寻找并鉴定在膀胱癌及癌旁组织中差异表达的微RNA（miRNA）。方法结合miRNA芯片技术寻找膀胱癌及癌旁组织中差异表达的miRNA,并通过荧光定量PCR进行验证。结果在癌与癌旁组织中共检测到115条存在显著性差异的miRNA,其中膀胱癌组织中下调42条,上调73条。结论膀胱癌组织与癌旁组织中miRNA表达谱存在较大差异,miRNA与膀胱癌的发生发展存在一定的相关性。
|
| 关 键 词： | 膀胱肿瘤 微RNA 聚合酶链反应 基因,肿瘤抑制 |
Differentially expressed miRNA in bladder cancer |
| |
| XIA Wei,SONG Tao,LI Jie,HUANG Bao-chun,LI Shao-hua,DING Hong-mei,WANG Tian-you,SHAO Ning-sheng. Differentially expressed miRNA in bladder cancer[J]. Bulletin of the Academy of Military Medical Sciences, 2010, 34(6): 540-542 |
| |
| Authors: | XIA Wei SONG Tao LI Jie HUANG Bao-chun LI Shao-hua DING Hong-mei WANG Tian-you SHAO Ning-sheng |
| |
| Affiliation: | 1.Beijing Institute of Basic Medical Sciences,Beijing 100850,China;2.Department of Urology,Chinese PLA General Hospital,Beijing 100853,China;3.Capital Institute of Pediatrics,Beijing 100020,China) |
| |
| Abstract: | Objective To identify the differentially expressed microRNA（miRNA） in bladder cancer.Methods The miRNA was detected by miRNA array and examined by qRT-PCR.Results A total of 115 differentially expressed miRNAs were detected by miRNA array in clinical bladder cancer and paracancer tissues,42 of which were low-expressed while 73 of them were highly-expressed in bladder cancer tissues.Conclusion The miRNA expression profile is different in bladder cancer and paracancer tissues,suggesting that miRNA might be involved in the initiation and development of bladder cancer. |
| |
| Keywords: | urinary bladder neoplasms microRNA polymerase chain reaction genes tumor suppressor |
| 本文献已被 维普 万方数据 等数据库收录！ |
|
