成年大鼠周围神经损伤后远侧端雪旺细胞变化的体外研究

目的:体外观察大鼠坐骨神经损伤不同时间段远侧端雪旺细胞的变化。方法:切断成年SD大鼠坐骨神经,以远侧端为研究对象,术后1、2周和1个月取材,进行雪旺细胞体外培养,倒置相差显微镜,S-100免疫荧光染色及激光扫描共聚焦显微镜观察雪旺细胞的形态,MTT检测细胞活力,流式细胞仪检测细胞周期。结果:神经被切断后,雪旺细胞形态发生改变,1月组最明显;细胞活力和增殖能力也发生变化,切断1周组活力和增殖能力最强,随后逐渐变弱。结论:周围神经损伤后雪旺细胞形态发生变化,细胞的活力和增殖能力随着时间的推移逐渐下降但仍未停止增殖。

Changes of Schwann cells in transected sciatic nerve distal stumps of adult rats in vitro

Objective: To study the changes in different time of Schwann cell in transected sciatic nerve distal stump. Methods: The sciatic nerves were cut at the middle level. Schwann cells (SCs) were obtained from the distal stumps of transected sciatic nerves which had been denervated for 1, 2 weeks and 1 month. The light microscope and loser scanning confocal microscope were used to observe morphological changes. MTT assay was conducted to detect the vitality of SCs. Flow cytometer was used to determine the changes in cell cycle. Results: Some morphological changes were observed especially in the group which had been denerved for 1 month. Besides, there was a progressive decrease in vitality and proliferation rate of SCs with increasing time of denervation. Conclusion: In trauma nerve, there were changes in morphology, vitality and proliferation rate. The longer the time of denervation was, the lower the vitality and proliferation ability of SCs was, however, the SCs do not stop proliferating though they have been denerved for 1 month.