年龄或性别对循环内皮祖细胞数量的影响

目的：研究年龄或性别对循环内皮祖细胞（cEPCs）数量的影响。方法：220例符合标准的健康成年志愿者均来自上海交通大学附属瑞金医院体检中心。所有志愿者根据年龄分为四组：〈30岁组（n=54）;30～49岁组（n=62）;50～69岁组（n=59）;〉69岁组（n=45）。每个年龄组志愿者按性别再分组。以CD34＋、CD133＋、VEGFR-2＋为表型标志,采用流式细胞学方法检测并对比志愿者cEPCs数量。结果：220例志愿者cEPCs数量不符合正态分布,因而取相应的自然对数值,使其符合正态分布。相同性别志愿者或全部志愿者的cEPCs数量自然对数值在不同年龄组组间无显著性差异（男性：F=1.234,P=0.301;女性：F=0.274,P=0.844;全部：F=1.078,P=0.359）。同时,在各年龄组或全部志愿者,男性和女性的cEPCs数量自然对数值之间无显著性差异（〈30岁组：t=0.699,P=0.488：30～49岁组：t=0.772,P=0.443;50～69岁组：t=-0.516,P=0.608;〉69岁组：t=-0.480,P=0.634;全部：t=0.216,P=0.829）。结论：年龄或性别对cEPCs数量没有影响。

Effect of age or gender on circulating endothelial progenitor cells number

Objective： To study the effect of age or gender on circulating endothelial progenitor cells（cEPCs） number.Methods： 220 standards-compliant healthy adult Chinese people were selected from the Health Examination Center of Ruijin Hospital Affiliated of Shanghai Jiaotong University.All subjects were divided into four different groups based on age（30 years group of 54 cases;30-49 years group of 62 cases;50-69 years group of 59 cases;and 69 years group of 45 cases.Meanwhile,subjects were subdivided by gender in each age group.The numbers of cEPCs（identified by flow cytometry as CD133＋,CD34＋ and VEGFR-2＋ cells） were detected and contrasted among different groups.Results： Data of cEPCs counts of 220 subjects were not in normal distribution,so data transformation was undertaken with calculating the corresponding natural logarithm.The natural logarithms of cEPC counts showed no notable difference among age group in male subjects（F=1.234,P=0.301）,female subjects（F=0.274,P=0.844） and all subjects（F=1.078,P=0.359）.Meanwhile,there was no significant difference of cEPCs counts′ natural logarithms between two genders in each age group and in all subjects（30 years group： t=0.699,P=0.488;30-49 years group： t=0.772,P=0.443;50-69 years group： t=-0.516,P=0.608;69 years group： t=-0.480,P=0.634;all subjects： t=0.216,P=0.829）.Conclusion： Age or gender are not associated with the level of cEPCs.