头部电针透穴对帕金森病模型大鼠黑质神经生长因子表达的影响

目的:探讨头部电针透穴疗法对帕金森病模型大鼠的保护作用机制.方法:健康Wistar大鼠36只随机分为正常组、假手术组、模型组和透针组,每组9只.其中假手术组于左侧纹状体微量注射生理盐水;模型组和透针组以6-羟基多巴胺左侧纹状体微量注射法制备偏侧帕金森病大鼠旋转模型;透针组在模型制备成功后采用头部电针透穴疗法进行治疗,穴取"百会""太阳",由"百会"沿皮向"太阳"透刺,接电针仪,每日治疗1次,6日为一疗程,共治疗2个疗程;其他组不予治疗干预.疗程结束后各组大鼠:(1)以免疫组织化学方法检测酪氨酸羟化酶(TH)阳性细胞的形态、数量;(2)以原位杂交技术检测脑源性神经生长因子(BDNF)mRNA的表达.结果:(1)透针组大鼠黑质多巴胺能神经元TH阳性表达面密度、数密度及积分光密度分别为0.065±0.011、0.014±0.003、0.470±0.099,模型组分别为0.039±0.008、0.008±0.002、0.266±0.065,透针组与模型组比较大鼠黑质多巴胺能神经元TH阳性表达面密度、数密度及积分光密度均增高(均P＜0.05);(2)透针组大鼠黑质BDNF mRNA表达的面密度、数密度及积分光密度分别为0.100±0.012、0.014±0.003、1.158±0.130,模型组分别为0.047±0.012、0.007±0.001、0.602±0.108,透针组与模型组比较BDNF mRNA表达在面密度、数密度及积分光密度方面均增加(均P＜0.05).结论:头部电针透穴疗法对帕金森病模型大鼠黑质多巴胺能神经元有较好的保护作用,其机制可能与激发BDNF的神经营养作用,进而改善多巴胺能神经元形态、增加多巴胺能神经元数量有关.

Effects of penetration therapy with scalp electroacupuncture on gene expressions of nerve growth factors in substantia nigra of rats with Parkinson's disease

Objective To explore the protective mechanism of penetration therapy with scalp electroacupuncture on Parkinson's disease(PD).Methods Thirty-six Wistar rats were randomly divided into a normal group,a sham operation group,a model group and a penetration therapy group,9 rats in each group.The sham operation group was operated by micro-injection with normal saline in the left corpus striatum.The model group and penetration therapy group were generated by micro-injection with 6-hydroxydopamine in the left corp...