大鼠气管上皮耐受热缺血时限的研究

目的 探讨肺移植无心跳供者气管上皮耐受热缺血的时限.方法 将SD大鼠分为4组.对照组在大鼠有心跳的情况下取出气管,热缺血30 min组、45 min组和60 min组分别在处死大鼠后30、45和60 min时取出气管,然后将供者气管固定后分别植入相应受者的大网膜内.14 d后,取出气管,进行病理学检查,并对上皮再生情况进行评分.结果 热缺血30min组和对照组比较,无论是上皮厚度还是黏膜上皮细胞层数的差异均无统计学意义(P>0.05);虽然45 min组与对照组相比,上皮厚度的差异有统计学意义(P<0.05),但45 min组的气管上皮有正常黏膜结构,并且与对照组相比,上皮细胞层数差异无统计学意义(P<0.05);而热缺血60min组,看不到正常的上皮结构,上皮也仅由1～2层黏膜上皮细胞覆盖.结论 气管耐受热缺血的时限可能为45 min.

Abstract：

Objective To determine the length of warm ischemic (WI) tolerance in bronchial graft from non-heart-beating donors. Methods Forty-eight rats were randomly divided into 4 groups (each group having 12 rats) according to different WI durations including WI-0 min (group A), WI-30 min (group B), WI-45 min (group C) and WI-60 min (group D). In each group, the tracheae from 6 rats were respectively imbedded in greater omentum of other 6 rats, and 14 days later, the transplanted tracheae were taken from recipients to evaluate epithelial thickness and regeneration. Results Epithelial thickness and the degree of epithelial regeneration had no significant difference (P >0. 05) between the syngeneic control group and the WI-30 minutes group. All of the grafts with WI duration of 45 min were viable, but the epithelium was significantly thinner than that in the syngeneic control group (P<0. 05). However all of the grafts with WI duration of 60 min showed lower viability rate. Conclusion The time limits of tolerance to WI of tracheal grafts from NHBDs may be 45 min.

Length of warm ischemic tolerance for epithelial regeneration in heterotopic rat tracheal isografts

Objective To determine the length of warm ischemic (WI) tolerance in bronchial graft from non-heart-beating donors. Methods Forty-eight rats were randomly divided into 4 groups (each group having 12 rats) according to different WI durations including WI-0 min (group A), WI-30 min (group B), WI-45 min (group C) and WI-60 min (group D). In each group, the tracheae from 6 rats were respectively imbedded in greater omentum of other 6 rats, and 14 days later, the transplanted tracheae were taken from recipients to evaluate epithelial thickness and regeneration. Results Epithelial thickness and the degree of epithelial regeneration had no significant difference (P >0. 05) between the syngeneic control group and the WI-30 minutes group. All of the grafts with WI duration of 45 min were viable, but the epithelium was significantly thinner than that in the syngeneic control group (P<0. 05). However all of the grafts with WI duration of 60 min showed lower viability rate. Conclusion The time limits of tolerance to WI of tracheal grafts from NHBDs may be 45 min.