ω-3多不饱和脂肪酸对创伤性休克大鼠急性肺损伤的影响

目的 探讨ω-3多不饱和脂肪酸(ω-3 PUFA)对创伤性休克大鼠急性肺损伤的影响.方法 雄性Wistar大鼠36只,体重240～260 g,月龄3月,采用随机数字表法,将大鼠随机分为3组(n=12):对照组(C组);创伤性休克组(TS组)采用骨折复合失血致创伤性休克;ω-3 PUFA组于造模前12 h及2 h经尾静脉注射ω-3 PUFA 2 ml/kg,C组和TS组注射生理盐水2 ml/kg.模型制备成功后120 min时采集颈动脉血样,处死取肺组织.用ELISA法检测血清TNF-α、8-异前列腺素F2α(8-iso-PGF2α)、IL-1β及IL-10的浓度;计算肺组织湿/干重比(W/D比);光镜下观察肺组织病理变化,行病理学评分.结果 与C组比较,TS组和ω-3 PUFA组血清TNF-α、8-iso-PGF2α、IL-1β及IL-10的浓度、肺组织W/D比及病理学评分明显升高(P＜0.01).与TS组比较,ω-3 PUFA组血清TNF-β、8-iso-PGf2α及IL-1β的浓度、肺组织W/D比及病理学评分明显降低,血清IL-10浓度升高(P＜0.05或0.01).结论 ω-3 PUFA可有效地抑制创伤性休克大鼠全身炎性反应,减轻急性肺损伤.

Abstract：

Objective To investigate the effects of ω-3 polyunsaturated fatty acid (ω-3 PUFA) on acute lung injury (ALI) in a rat model of traumatic shock. Methods Thirty-six male Wistar rats aged 3 months were randomly assigned into 3 groups ( n = 12 each): control group (group C) ; traumatic shock group (group TS) and ω-3 PUFA + TS group (group to-3 PUFA) . Traumatic shock was induced by fracture of femur and hemorrhage according to the method described by Feeney in groups TS and ω-3 PUFA. In group ω-3 PUFA, ω-3 PUFA 2 ml/kg was injected via the caudal vein at 12 and 2 h before induction of traumatic shock. Arterial blood samples were taken at 120 min after traumatic shock was successfully induced for determination of serum concentrations of TNF-α, IL-1β, IL-10 and 8-iso-PGF2α by ELISA. The animals were then sacrificed and lungs were removed for-determination of W/D lung weight ratio and microscopic examination. Results Traumatic shock significantly increased serum concentrations of TNF-α, IL-1β, IL-10 and 8-iso-PGF2α, W/D ratio and pathologic scores of lung tissues in groups TS and ω-3 PUFA as compared with group C.ω-3 PUFA significantly attenuated traumatic shuck-induced increase in serum concentrations of TNF-α, IL-1β and 8-iso-PGF2α , W/D ratio and pathologic scores of lung tissues but further increased the serum IL-10 concentration in group ω-3 PUFA as compared with group TS. Conclusion ω-3 PUFA can significantly inhibit the svstemic inflammatory response and ameliorate traumatic shock-induced ALI.