| 电针"内关"穴预处理对缺血再灌注大鼠心肌阿片受体基因表达的影响 |
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| 作者姓名： | Li WS Zhong M Yang JH Zhao WX |
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| 作者单位： | 1. 广州中医药大学第二附属医院,广东广州,510120
2. 广州市越秀区正骨医院 |
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| 基金项目： | 广东省中医药局资助项目 |
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| 摘 要： | 目的:探讨"内关"穴预处理对心脏保护的效应及作用机制.方法:健康雄性Wistar大鼠48只,随机分为假手术组(N组)、心肌缺血模型组(M组)、电针组(E组).M组采用结扎左冠状动脉前降支(LAD)30 min、再灌注120 min的方法制备心肌缺血再灌注模型;N组只开胸穿线,不结扎LAD;E组在缺血前3 d每天给予双"内关"穴电针刺激20 min.用TTC染色的方法测定大鼠心梗面积;用生化方法测定血中肌酸激酶同工酶(CK-MB)及乳酸脱氢酶(LDH)含量;光镜下观察心肌形态学改变;应用实时荧光定量RT-PCR法测定心肌δ阿片受体(DOR)和K阿片受体(KOR)mRNA的表达.结果:E组较M组心肌梗死面积明显缩小(P＜0.05).M组CK-MB 和LDH水平分别为(980±92)U/L和(2 743±124)U/L,均较N组的(312±41)U/L和(530±56)U/L显著升高(均P＜0.01),E组的CK-MB及LDH水平分别为(572±70)U/L和(1819±97)U/L,均较M组明显降低(均P＜0.01).DOR和KOR表达在M组与N组间无明显差别(均P＞0.05),E组D0R表达较M组和N组显著增加(均P＜0.01),KOR表达在3组间差异无统计学意义(均P＞0.05).结论:电针"内关"穴预处理可通过上调心肌δ阿片受体基因表达对缺血再灌注心肌产生保护作用.
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| 关 键 词： | 电针 穴 内关 缺血再灌注损伤 心肌 受体 阿片 |
Effects of electroacupuncture preconditioning at "Neiguan" (PC 6) on gene expression of myocardial opioid receptors in rats with myocardial ischemia-reperfusion injury |
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| Li WS,Zhong M,Yang JH,Zhao WX.Effects of electroacupuncture preconditioning at "Neiguan" (PC 6) on gene expression of myocardial opioid receptors in rats with myocardial ischemia-reperfusion injury[J].Chinese Acupuncture & Moxibustion,2011,31(5):441-445. |
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| Authors: | Li Wan-Shan Zhong Min Yang Jin-Hui Zhao Wei-Xian |
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| Institution: | LI Wan-shan1,ZHONG Min1,YANG Jin-hui2,ZHAO Wei-xian1(1.The Second Affiliated Hospital of Guang-zhou University of TCM,Guangzhou 510120,Guangdong Province,China,2.Guangzhou Yuexiu District Osteopathy Hospital) |
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| Abstract: | Objective To explore the protective effects of electroacupuncture preconditioning at Neiguan(PC 6) on myocardial ischemia reperfusion injury(MIRI) and the mechanism.Methods Forty-eight male Wistar rats were randomly divided into a sham operation group(group N),a MIRI group(group M) and an electroacupuncture group(group E).The MIRI model was generated by ligating the left anterior descending artery(LAD) for 30 min followed by reperfusion for 120 min.The partition sutures in group N were passed under the LAD ... |
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| Keywords: | Electroacupuncture Point PC 6(Neiguan) Ischemia Reperfusion Injury Myocardial Receptor Opioid |
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