拮抗或激活促肾上腺皮质激素释放因子受体对肠易激综合征大鼠内脏敏感性及结肠动力的影响

目的 探讨促肾上腺皮质激素释放因子(CRF)及其受体对肠易激综合征大鼠内脏敏感性及结肠动力的影响.方法 SD大鼠60只,随机平均分入空白组(不做处理)、模型组(特殊气味条件刺激和肢体束缚直肠刺激非条件刺激轮替致敏)、干预对照组(造模前侧脑室注射0.9%NaC1)、干预一组(造模前侧脑室注射CRF-R1拮抗剂)和干预二组(造模前侧腑室注射CRF-R2激动剂).采用腹部收缩反射(AWR)评分标准评估各组大鼠肠道敏感性,记录各组大鼠结肠快、慢波波动率、最大振幅、收缩波数及振幅指数等电生理活动改变.采用SPSS16.0统计软件分析,计量资料采用方差分析,等级资料采用秩和检验.结果 以AWR=3分时所需的直肠注水量作为评价指标,模型组大鼠(0.90±0.11)ml]较空白组(1.23±0.07)ml]内脏敏感性增高(F＝82.586,P＜0.01);结肠电生理活动增强,造模成功.干预对照组直肠注水量为(0.81±0.11)ml,与模型组(0.90±0.11)ml]差异无统计学意义(F＝3.734,P＞0.05),干预一组(1.28±0.07)ml,F＝161.878,P＜0.01]和干预二组(1.22±0.05)ml,F＝121.564,P＜0.01]较干预对照组内脏敏感性降低.干预对照组大鼠结肠快、慢波波动率、最大振幅、收缩波数及振幅指数等电生理活动与模型组无明显差异(P均＞0.05);干预一组和干预二组大鼠结肠电生理活动均较干预对照组明显减弱(均P＜0.05).结论 CRF在IBS发病中起重要作用,抑制CRF-R1或激活CRF-R2可降低1BS大鼠内脏敏感性并抑制结肠运动.

Abstract：

Objective To explore the effect of corticotropin releasing factor (CRF) and its receptor on visceral sensitivity and colon motility of irritable bowel syndrome (IBS) rats. Methods sixty SD rats were divided randomly and equally into control group (without treatment),model group (sensitized in turn with camphor odor as conditional stimulation and physical restraint in combination with rectal distention pressure as non-conditional stimulation),treatment control group (injected physiological saline into lateral ventricles before treatment),treatment group 1 (injected CRF-R1antagonist into lateral ventricles before treatment),treatment group 2 (injected CRF-R2 agonist into lateral ventricles before treatment). Then the rats' visceral sensitivity were assessed by AWR,and colonic electricity activities such as volatility,maximum amplitude of fast wave and slow wave,interdigestive number of contraction wave and index of contraction were recorded. The data was analyzed with SPSS 16. 0 software. Results By the amount of ractal water injection to reach AWR=3 as the evaluation index,model group (0. 90±0. 11) ml] showed higher visceral sensitivity than that of control group (1. 23±0. 07) ml,F=82. 586,P＜0. 01],and colonic electricity activity increased (P＜0. 05),model was successfully set up. There was no significant difference of the amount of ractal water injection between model group (0. 90±0. 11) ml] and treatment control group (0. 81±0. 11) ml,F=3. 734,P＞0. 05]. Compared with treatment control group,the visceral sensitivity decreased in treatment group 1 (1. 28±0. 07) ml,F=161. 878,P＜0. 01] and treatment group 2 (1. 22±0.05) ml,F=121. 564,P＜0. 01]. There was no significant difference between treatment control group and model group in electricity activities such as volatility,maximum amplitude of fast wave and slow wave,interdigestive number of contraction wave and index of contraction (all P＞0. 05). While the electricity activities was weakened in treatment group 1 and 2 compared with the treatment control group (all P＜0. 05). Conclusions CRF plays an important role in the pathogenesis of IBS. Inhibition of CRF-R1 or activation of CRF-R2 may lower visceral hypersensitivity and decrease colon motility of rats.

The effect of antagonizing corticotropin releasing factor receptor 1/activating corticotropin releasing factor receptor 2 on visceral sensitivity and colonic motility of irritable bowel syndrome rats

Objective To explore the effect of corticotropin releasing factor (CRF) and its receptor on visceral sensitivity and colon motility of irritable bowel syndrome (IBS) rats. Methods sixty SD rats were divided randomly and equally into control group (without treatment),model group (sensitized in turn with camphor odor as conditional stimulation and physical restraint in combination with rectal distention pressure as non-conditional stimulation),treatment control group (injected physiological saline into lateral ventricles before treatment),treatment group 1 (injected CRF-R1antagonist into lateral ventricles before treatment),treatment group 2 (injected CRF-R2 agonist into lateral ventricles before treatment). Then the rats' visceral sensitivity were assessed by AWR,and colonic electricity activities such as volatility,maximum amplitude of fast wave and slow wave,interdigestive number of contraction wave and index of contraction were recorded. The data was analyzed with SPSS 16. 0 software. Results By the amount of ractal water injection to reach AWR=3 as the evaluation index,model group (0. 90±0. 11) ml] showed higher visceral sensitivity than that of control group (1. 23±0. 07) ml,F=82. 586,P＜0. 01],and colonic electricity activity increased (P＜0. 05),model was successfully set up. There was no significant difference of the amount of ractal water injection between model group (0. 90±0. 11) ml] and treatment control group (0. 81±0. 11) ml,F=3. 734,P＞0. 05]. Compared with treatment control group,the visceral sensitivity decreased in treatment group 1 (1. 28±0. 07) ml,F=161. 878,P＜0. 01] and treatment group 2 (1. 22±0.05) ml,F=121. 564,P＜0. 01]. There was no significant difference between treatment control group and model group in electricity activities such as volatility,maximum amplitude of fast wave and slow wave,interdigestive number of contraction wave and index of contraction (all P＞0. 05). While the electricity activities was weakened in treatment group 1 and 2 compared with the treatment control group (all P＜0. 05). Conclusions CRF plays an important role in the pathogenesis of IBS. Inhibition of CRF-R1 or activation of CRF-R2 may lower visceral hypersensitivity and decrease colon motility of rats.