p53、p21ras、nm23-H1基因在子宫内膜癌中表达与临床病理关系研究

 目的　研究p53、p21ras、nm23-H1基因在子宫内膜癌中的表达与临床病理学关系。方法　应用免疫组织化学方法(SABC法)研究18例正常增生期子宫内膜、12例子宫内膜非典型性增生过长、78例子宫内膜癌的p53、p21ras、nm23-H1基因的表达情况。结果　12例增生期子宫内膜中p53和p21ras基因表达阴性，3例子宫内膜非典型性增生过长中p53阳性；p53、p21ras、nm23基因在子宫内膜癌中表达率分别为57.69%，55.12%，71.79%；p53的表达在不同的临床分期，病理学分级，有无淋巴结转移和是否绝经之间差异有显著性意义(P<0.01)；p21ras基因表达与病理学分级及有无淋巴结转移有关(P<0.05)，但与临床分期无关；nm23-H1的表达与病理学分级，临床分期，有无淋巴结转移有关。结论　p53、p21ras、nm23基因在子宫内膜癌中均高表达，p53的表达和肿瘤发生，发展有关，p21ras基因表达与肿瘤的进展有关，nm23的表达与淋巴结转移呈负相关。

The Expression of p53,p21ras and nm23-H1 Gene in Endometrial Carcinoma and their Relationshop with Clinical Pathology

Objective To investigate the expression of p53,p21ras and nm23-H1 gene in endometrial carcinoma and their relationship with clinical pathology.Methods Immunohistochemical method(SABC) was used to study the expression of p53 p21ras and nm23 gene and their relationship with clinical pathology in 18 cases of normal proliferative endometrium,12 of atypical endometrium hyperplasia and 78 of endometrial carcinoma.Results 3 cases shows positive expression of p53.The expressions of p53,p21ras and nm23 in endometrial carcinoma are 57.69%，52.56%，71.79%，respectively.The expression of p53 has significant difference in various clinical stage,pathologic degree,and lymphoid node metastasis (P<0.01)；p21ras expression showed significant difference between the pathologic degree and lymphoid node metastasis (P<0.05)，which has no significant difference with clinical stage.The expression of nm23 has negative relation with lymph node metastasis.Conclusion The p53,p21ras and nm23 were highly expressed in endometrial carcinoma,and the expression of p53 relates to the pathogenesis and progression of endometrial carcinoma;p21ras expression was relative to the progression of the endometrium carcinoma.The expression of nm23 was relative to the lymphoid node metastasis