| 甲基汞急性暴露诱导大鼠内质网应激的脑区特异性及时间效应特征研究 |
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| 引用本文： | 张叶,蒋旭超,赵小武,钱海,王苏华,邢光伟,王世忠,陆荣柱.甲基汞急性暴露诱导大鼠内质网应激的脑区特异性及时间效应特征研究[J].卫生研究,2010,39(3). |
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| 作者姓名： | 张叶 蒋旭超 赵小武 钱海 王苏华 邢光伟 王世忠 陆荣柱 |
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| 作者单位： | 江苏大学基础医学与医学技术学院预防医学教研室,镇江,212013 |
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| 基金项目： | 国家自然科学基金项目(No.30872139); 江苏大学营养与疾病科研团队基金; 江苏大学大学生科研立项及实践创新训练计划项目 |
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| 摘 要： | 目的探讨甲基汞急性暴露诱导大鼠不同脑区内质网应激的时间-效应关系。方法42只SD大鼠随机分为7组,染毒组腹腔注射氯化甲基汞(4mg/kg),并在0.5、1、3、6、12和24h后麻醉断头处死。对照组腹腔注射同等剂量的生理盐水后立刻断头处死,迅速在冰上分离小脑、大脑皮层、脑干、海马和纹状体5个脑区。以蛋白印迹法测定各个脑区内质网应激的标志性蛋白Grp78的表达。同时测定大脑皮层内还原型谷胱甘肽(GSH)含量。结果甲基汞染毒后,各个脑区Grp78蛋白的表达显示了较为一致的先升高后降低的变化趋势,0.5h开始升高,6h或12h达到高峰,然后下降,24h时接近对照组水平,其中大脑皮层与脑干Grp78的变化与对照组相比分别具有统计学意义。大脑皮层Grp78蛋白的表达在染毒6h时处在最高峰,表达量与对照组相比增加约50%。脑干在染毒12h处在最高峰,表达量比对照组增加约40%。GSH的测定结果显示,与对照组相比,大脑皮层GSH含量呈先降低后缓慢升高的趋势,显示出与Grp78变化相反的趋势。结论甲基汞急性暴露大鼠显示内质网应激具有时间效应和脑区特异性,这种内质网应激效应可能与氧化性应激有关。
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| 关 键 词： | 甲基汞 内质网应激 Grp78蛋白 还原型谷胱甘肽 |
Time-course effect and region-specificity of endoplasmic reticulum stress in rat brains acutely exposed by methylmercury |
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| ZHANG Ye,JIANG Xuchao,ZHAO Xiaowu,QIAN Hai,WANG Suhua,XING Guangwei,WANG Shizhong,LU Rongzhu.Time-course effect and region-specificity of endoplasmic reticulum stress in rat brains acutely exposed by methylmercury[J].Journal of Hygiene Research,2010,39(3). |
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| Authors: | ZHANG Ye JIANG Xuchao ZHAO Xiaowu QIAN Hai WANG Suhua XING Guangwei WANG Shizhong LU Rongzhu |
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| Institution: | ZHANG Ye,JIANG Xuchao,ZHAO Xiaowu,QIAN Hai,WANG Suhua,XING Guangwei,WANG Shizhong,LU Rongzhu Department of Preventive Medicine in Basis Medicine , Medical Technology Institution,Jiangsu University,Zhenjiang 212013,China |
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| Abstract: | Objective To explore time-course effect and region-specificity of endoplasmic reticulum stress in rat brain acutely exposed by methylmercury(MeHg).Methods Forty-two SD rats were randomly divided into seven groups,and the rats intraperitoneally injected at the dose of 4 mg /kg bw.MeHg were decapitated at the times of 0.5,1,3,6,12 and 24 h,then rats in control group intraperitoneally injected by the corresponding volume of normal saline were decapitated.The cerebellum,cerebral cortex,brain stem,hippocampus an... |
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| Keywords: | rat methylmercury endoplasmicreticulum stress Grp78 glutathione |
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