| 三元络合物比色法测定复方苦参洗剂中小檗碱的含量 |
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| 引用本文： | 龙小华,厉辉,黄嵘. 三元络合物比色法测定复方苦参洗剂中小檗碱的含量[J]. 药学实践杂志, 2004, 22(5): 294-296 |
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| 作者姓名： | 龙小华 厉辉 黄嵘 |
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| 作者单位： | 长沙市第四医院,湖南,长沙,410006 |
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| 摘 要： | 目的:建立三元络合物比色法测定复方苦参洗剂中小檗碱的含量,为制剂质量控制提供依据。方法:小檗碱在pH值为6.7的溶液中,可与溴酚蓝、奎宁形成三元络合物,经氯仿提取,在610nm波长处测定吸收度。结果:小檗碱在0.8～4mg/L范围有良好的线性关系(r=0.999 9),平均回收率为98.65％,RSD为1.12％。结论:本方法准确,灵敏度好,可用于复方苦参洗剂等中药制剂中小檗碱的含量测定。
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| 关 键 词： | 复方苦参洗剂 小檗碱 三元络合物比色法 含量测定 |
| 文章编号： | 1006-0111(2004)05-0294-03 |
| 收稿时间： | 2003-11-17 |
| 修稿时间： | 2003-11-17 |
Determination of bererine in compound Sophora flavescens lotions by three variables complex chromatometry |
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| LONG Xiao-hu,LI Hui and HUANG Rong. Determination of bererine in compound Sophora flavescens lotions by three variables complex chromatometry[J]. The Journal of Pharmaceutical Practice, 2004, 22(5): 294-296 |
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| Authors: | LONG Xiao-hu LI Hui HUANG Rong |
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| Affiliation: | The Fourth Hospital of Changsha, Changsha 410006, China;The Fourth Hospital of Changsha, Changsha 410006, China;The Fourth Hospital of Changsha, Changsha 410006, China |
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| Abstract: | Objective: To establish three variables complex chromometry for the determination of berberine in compound Sophora flavescens lotions. Methods:The berberine formed three variables complex with bromophenol blue and quinine in pH6.7,then it was withdrawn with chloroform. The detection wavelength was 610nm. Results: The linear range of berberine was the 0. 8 ~ 4mg/L( r = 0. 999 9) ,the average recovery was 98.65% ,RSD was 1. 12% . Conclusion:The method had a good sensitive, and reproducibility. It can be used for determination of berberine in compound sophora flavescens lotions. |
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| Keywords: | compound Sophora flavescens lotions berberine three variables complex chromatometry determination of content |
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