| CDKN 2/p16基因甲基化失活与肺癌关系的研究 |
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| 引用本文： | 苏长青,单祥年.CDKN 2/p16基因甲基化失活与肺癌关系的研究[J].中华医学遗传学杂志,2000,17(1):36-38. |
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| 作者姓名： | 苏长青 单祥年 |
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| 作者单位： | 1. 210002,南京,南京八一医院全军肿瘤研究中心
2. 南京师范大学生命科学学院 |
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| 基金项目： | 国家九五重点科技项目(969060118)；国家自然科学基金项目(39670714) |
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| 摘 要： | 目的 研究ＣＤＫＮ２／ｐ１６基因５’端调控序列区ＣｐＧ岛甲基化的状态与肺癌发展的关系。方法 采用甲基化敏感性核酸酶切基因组ＤＮＡ的方法，对８９例肺癌的ＣＤＳＮ２／ｐ１６基因进行了Ｓｏｕｔｈｅｒｎ杂交分析。结果 ８９例肺癌发现该基因甲基化２１例，甲基化频率为２３．６％（２１／８９），其中１７例发生于４２例Ｐ１６蛋白 阴性表达的患者。结论 ＣＤＫＮ２ｐ／１６基因５’端ＣｐＧ岛甲基化可能是该基因失活的重
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| 关 键 词： | 肺肿瘤 CDSN2／P16基因 甲基化失活 核酸内切酶 |
| 修稿时间： | 1999-01-11 |
Correlation of inactivation of CDKN2/p16 gene by methylation and lung cancers |
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| SU Changqing,SHAN Xiangnian.Correlation of inactivation of CDKN2/p16 gene by methylation and lung cancers[J].Chinese Journal of Medical Genetics,2000,17(1):36-38. |
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| Authors: | SU Changqing SHAN Xiangnian |
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| Institution: | Department of Molecular Biology, Nanjing 81 Hospital, Nanjing, Jiangsu, 210002 P.R. China. sucq@jlonline.com |
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| Abstract: | OBJECTIVE: To study the relationship between methylation state of CDKN2/p16 gene and lung cancers. METHODS: Genomic DNA was digested with methylation-sensitive enzymes and Southern blotting was used to analyses the methylation state of CDKN2/p16 gene in 89 cases of lung cancer. RESULTS: Twenty-one out of 89 lung cancers(23.6%) shown methylation of CDKN2/p16 gene, and 17 of them occurred in 42 P16 negative cases with a rate of 40.5%(17/42). CONCLUSION: Methylation of CDKN2/p16 gene 5' CpG island is probably an important mechanism of gene inactivation; it may take part in the onset and process of lung cancer. |
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| Keywords: | lung neoplasm CDKN2/p16 gene methylation restriction endonuclease Southern blotting |
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