The effect of oxytetracycline on the response to insulin of diaphragm muscle and on lipid synthesisin vivo andin vitro in the ob/ob mouse

Summary The insulin sensitivity of the peripheral tissues in ob/ob mice treated with oxytetracydine was assessed bothin vivo andin vitro by measuring the effect of insulin on the incorporation of glucose into glycogen in diaphragm muscle and on the incorporation of glucose and³H₂O into lipids in epididymal adipose tissue. The results indicated that OTC treatment improved the insulin sensitivity of muscle but not that of adipose tissue. The results presented also indicated that oxytetracycline treatment led to a decrease in the basal incorporation of lipids into the liverin vivo and the adipose tissuein vivo andin vitro. The results are discussed in view of the relative importance of liver and adipose tissue in the lipid metabolism of ob/ob and lean mice and in view of results obtained by other workers on the effects of B -cell cytotoxic agents.