应用6种增菌液5种培养基检测8种常见食源性致病菌的报告

目的　快速全面地检测常规细菌性食物中毒致病菌 ,为临床提供实验室诊断依据。方法　依据《食品卫生检验方法》微生物学部分GB 4789 1-94,采用 6种增菌液 5种培养基就可完成 8种常见致病菌的检验工作。结果　 2 0 0 3年深圳市龙岗区发生的 9宗食物中毒 ,临床诊断为细菌性食物中毒的有 6宗 ,其中 5宗均分离出致病菌。 45份食物中毒送检标本中 ,共分离出致病菌 11株 ,分离率 2 4 4%。菌型有金黄色葡萄球菌 2株 ,O 15 7;H 7大肠埃希菌 2株 ,甲型副伤寒 3株 ,福氏Ⅱ型志贺氏菌 2株 ,副溶血性弧菌 2株。结论　应用 6种增菌液 5种培养基能快速检测 8种常见食源性致病菌。

Application of 6 kinds of proliferous liquids and 5 kinds of culture mediums in testing 8 kinds of food - induced pathogenic bacteria

Objective To rapidly examine the overall routine pathogenic bacteria and provide basis for clinical diagnosis. According to the microbiology parts GB 4789.1-94 of "Methods for Food Sanitation Examination",the application of 6 kinds of proliferous liquids and 5 kinds of culture mediums is effective in testing 8 kinds of pathogenic bacteria. 11 strains of pathogenic bacteria were separate from 45 samples of food poisoning (24.4%). 2 of the strains were staphylococcus aureus,2 were H7 Escherichia coli,3 were type A Bacillus paratyphosus,2 were Shiga's bacillus,2 were Vibrio parahaemolyticus. [Conclusion] The application of 6 kinds of proliferous liquids and 5 kinds of culture mediums is an effective way to test 8 kinds of food-induced pathogenic bacteria.