| 体外培养的人牙乳头间充质细胞的矿化特征 |
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| 引用本文： | 陈新梅,肖明振,倪龙兴,刘军. 体外培养的人牙乳头间充质细胞的矿化特征[J]. 牙体牙髓牙周病学杂志, 2000, 10(1): 10-10 |
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| 作者姓名： | 陈新梅 肖明振 倪龙兴 刘军 |
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| 作者单位： | 第四军医大学口腔医学院,陕西,西安,710032 |
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| 摘 要： | 目的；观察体外２的人牙乳头间充质细胞矿化特征，深入研究成牙本质细胞的分化和牙本质形成。方法：第４代人牙也头间充质细胞连续培养３５ｄ后，进行组织学染色，透射电镜和扫描电镜观察。结果：人牙乳头间质细胞可出现复层生长和形成矿化结节，ｖｏｎ Ｋｏｓｓａ杂色提示细胞结节中有明显的钙盐沉积；细胞可出现与成牙本质细胞相似的超微结构特征并出现典型的矿化影像。
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| 关 键 词： | 牙乳头 间充质 基质小泡 DPMC 矿化特征 |
| 修稿时间： | 1999-07-05 |
The calcification ability of cultured human dental papilla mesenchymal cells |
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| CHEN Xin-mei,XIAO Ming-zhen,NI Long-xing,et al School of Stomatology,The Fourth Military Medical University,Xi'an $$$$. The calcification ability of cultured human dental papilla mesenchymal cells[J]. Chinese Journal of Conservative Dentistry, 2000, 10(1): 10-10 |
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| Authors: | CHEN Xin-mei XIAO Ming-zhen NI Long-xing et al School of Stomatology The Fourth Military Medical University Xi'an $$$$ |
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| Affiliation: | CHEN Xin-mei,XIAO Ming-zhen,NI Long-xing,et al School of Stomatology,The Fourth Military Medical University,Xi'an 710032$$$$ |
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| Abstract: | AIM: Observation of the calcification ability of cultured human dental papilla mesenchymal cells(DPMC). METHODS: The fourth passage human DPMC were cultured up to 35d. Observed by transmission electron microscopy(TEM), scanning electron microscopy(SEM) and light microscope(LM). RESULTS: The cells presented mineralized nodules as well as matrix vesicles and had same ultrastructure with odontoblasts under TEM. CONCLUSION: The results suggested that cultured human DPMC have the calcification ability which were probably related to matrix vesicles. |
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| Keywords: | dental papilla mesenchymal matrix vesicle |
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