Autoantibodies against spermatozoal antigens detected by immunoblotting and agglutination. A longitudinal study of vasectomized males |
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| Authors: | J Hald S Naaby-Hansen J Egense T Hjort O J Bjerrum |
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| Affiliation: | 1. Department of Political Sciences, University of Pretoria, South Africa;2. Hot or Cool Institute, Berlin, Germany;3. Crawford School of Public Policy, The Australian National University, Australia;4. Wellbeing Economy Alliance, Glasgow, UK;5. Environmental Planning and Climate Protection Department, eThekwini Municipality, Durban, South Africa;6. European Environment Agency, Copenhagen, Denmark;7. Department of Health Sciences, University of York, UK;8. Division of Epidemiology and Public Health, University of Nottingham, UK;9. Institutes of Earth Sciences and Sustainable Development Studies, University of Iceland, Iceland;10. University College London, UK;11. Natural Capitalism Solutions, Longmont, CO, United States;12. Department of Social Psychology and Development, University of Padova, Italy |
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| Abstract: | Sera taken pre- and post-operatively at regular intervals within a year from 16 men undergoing vasectomy were analysed for autoantibodies against spermatozoal proteins by immunoblotting. The reaction patterns were compared with the results of sperm agglutination tests. Immunoblotting revealed the presence of autoantibodies against various spermatozoal polypeptides in all sera taken pre-operatively and post-operatively. On average, seven polypeptides showed reaction. During the post-operative period two patients developed spermatozoal agglutinins in moderate titers (greater than 16) but in immunoblotting no change in band reactivity was observed for these two patients. However, scanning of the immunoblotting results revealed that one of the patients, although without sperm agglutinins, during the post-operative period showed an increasing band colouring of a polypeptide of Mr 31,500, reflecting an increased level of the corresponding antibodies. |
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