白细胞介素-6基因-174G/C多态性与阿尔茨海默病及血管性痴呆的关联研究

目的 探索白细胞介素-6(IL-6)基因启动子区-174G/C多态性与阿尔茨海默病(AD)、血管性痴呆(VD)的关系.方法 采取病例对照研究方法 ,以广州地区流行病学调查中诊断的161例AD、54例VD患者和247名健康老年人为研究对象,用聚合酶链反应-限制性片段长度多态性技术检测IL-6基因-174G/C多态性.结果 (1)所有研究对象均无C/C基因型.(2)AD组C等位基因频率(0.9%)及G/C基因型(1.9%)高于正常对照组(均为0),但差异无统计学意义(P＞0.05);按是否携带载脂蛋白E(Apo E)ε4进行分层,差异仍无统计学意义(P＞0.05).(3)VD患者C等位基因(1.9%)频率及G/C基因型(3.7%)高于正常对照组(均为0),差异有统计学意义(均P＜0.05).(4)中、重度AD患者含ε4等位基因的频率(23.9%)高于对照组(14.7%,P＜0.05).结论 IL-6基因-174G/C多态性不是广州地区汉族人群AD发病的危险因素,但与VD可能有关联.

Association analysis between the interleukin-6 gene promoter-174G/C polymorphism with Alzheimer disease and vascular dementia in Guangzhou

Objective To investigate the association between interleukin-6(IL-6)gene promoter-174G/C polymorphism with Alzheimer disease(AD)and vascular dementia(VD)in Han Chinese.Methods The subjects came from an epidemiological investigation.Association study was performed in 161 AD patients,54 VD patients and 247 healthy old individuals in Guangzhou,China.The-174C/G polymorphism of IL-6 gene promoter was analyzed with the technique of polymerase chain reaction-restriction fragment length polymorphism.Results (1)The genotype C/C wosn't detected in all subjects.(2)No significant difference in distribution of IL-6-174c/G polymorphism between AD patients and controls Was observed(P>0.05),even after stratification by Apo E ε4 status.(3)The frequencies of IL-6 genotype C/G(3.7%)and allele C(1.9%)in the VD patients were significant higher than that in controls(0)(Fisher's exact test,P<0.05).(4)The frequency of Apo E s-4 allele(23.9%)in moderate or severe AD patients was significant higher than controls(14.7,P<0.05).Conclusion The IL-6 gene-174C/G polymorphism may be not associated with AD,but possibly with VD.