| EFFECT OF PROCYANIDIN ON CELL CYCLE IN MURINE LEWIS LUNG CARCINOMA |
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| 引用本文： | 娄金丽,林洪生,邱全瑛,裴迎霞,祁鑫. EFFECT OF PROCYANIDIN ON CELL CYCLE IN MURINE LEWIS LUNG CARCINOMA[J]. 中国癌症研究, 2004, 16(3): 220-224. DOI: 10.1007/s11670-004-0031-9 |
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| 作者姓名： | 娄金丽 林洪生 邱全瑛 裴迎霞 祁鑫 |
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| 作者单位： | Department of Microbiology and Immunology，Beijing University of Traditional Chinese Medicine，Tumor Laboratory，Guanganmen Hospital，China Academy of Traditional Chinese Medicine，Department of Microbiology and Immunology，Beijing University of Traditional Chinese Medicine，Tumor Laboratory，Guanganmen Hospital，China Academy of Traditional Chinese Medicine，Tumor Laboratory，Guanganmen Hospital，China Academy of Traditional Chinese Medicine Beijing 100029，Beijing，100053，Beijing 100029，Beijing，100053，Beijing，100053 |
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| 基金项目： | ThisworkwassupportedbygrantfromTheStateAdministrationofTraditionalChineseMedicineofChina(No.2000-J-B-02). |
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| 摘 要： | Fagopyrumcymosum(Trev.)Meisnisaspeciesofbuckwheat.IthasbeenusedinChinaformedicinalpurposes.Themedicaleffectcomesfromitsroots.Chineseliteraturereportsthatthisplantcan揷learlungheat?and揹isseminatepoison?implyingthatitmayhaveanti-inflammatoryandanti-tumoreffects.ItwasreportedthattheFagopyrumcymosumextractcontainsprocyanidinedimmers(B2,B4andC2),catechins,epi-catechins(EC),(-)epigallocatechin(EGCG),hecogenin,quercetin,bete-sitosterol,P-coumaricacidandferulicacid[1].SomeactiveingredientsofFa…
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| 关 键 词： | 细胞周期 鼠科 肺癌 肿瘤 肺部疾病 |
| 收稿时间： | 2003-12-23 |
| 修稿时间： | 2004-06-22 |
Effect of procyanidin on cell cycle in murine lewis lung carcinoma |
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| Lou Jin-li,Lin Hong-sheng,Qiu Quan-ying,Pei Ying-xia,Qi Xin. Effect of procyanidin on cell cycle in murine lewis lung carcinoma[J]. Chinese Journal of Cancer Research, 2004, 16(3): 220-224. DOI: 10.1007/s11670-004-0031-9 |
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| Authors: | Lou Jin-li Lin Hong-sheng Qiu Quan-ying Pei Ying-xia Qi Xin |
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| Affiliation: | (1) Department of Microbiology and Immunology, Beijing University of Traditional Chinese Medicine, 100029 Beijing;(2) Tumor Laboratory, Guanganmen Hospital, China Academy of Traditional Chinese Medicine, 100053 Beijing |
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| Abstract: | Objective: To investigate the anti-tumor effect of procyanidin (WeiMaiNing, WMN) on a murine Lewis lung carcinoma cell line (3LL) and the influence on the cell cycle. Methods: The inhibitory rate of 3LL growth was detected in the model of murine Lewis lung carcinoma. The effect of the drug on 3LL cell cycle and the influence of the drug on the expression of cyclin D1 protein were investigated by flow cytometry, immunohistochemical staining. Results: The inhibitory rates of WMN in 3LL were 19.14%, 33.59% and 51.56% respectively at dosages 100 mg·kg−1·d−1, 150 mg·kg−1·−1 and 250 mg·kg−1·d−1. The inhibitory effect was in a dose-dependent manner. We found WMN significantly increased (P<0.01) the number of 3LL cells in G0–G1 phase (35.97% vs. 27.2% at 150 mg·kg−1·−1 WMN; 40.10% vs. 27.2% at 250 mg·kg−1·−1 WMN) and decreased the expression of cyclin D1, PCNA protein. Conclusion: WeiMaiNing inhibits the growth of 3LL cells in vivo by decreasing the expression of PCNA and cyclin D1, blocking the cells in G0–G1 phase and preventing the cells transition from G1 to S phase while DNA is replicated. Foundation item: This work was supported by grant from The State Administration of Traditional Chinese Medicine of China (No. 2000-J-B-02). Biography: LOU Jin-li (1964–), female, associate professor, candidate for doctor of medicine, Beijing University of Traditional Chinese Medicine, majors in immunology. |
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| Keywords: | Procyanidin Lewis lung carcinoma Cyclin D_1 PCNA |
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