6种中药挥发油体外抑杀人体蠕形螨活性及安全性研究

目的:筛选体外抗螨有效的中药挥发油。方法:采用透明胶带粘贴法获取蠕形螨,镜下观察不同中药挥发油对毛囊蠕形螨和皮脂蠕形螨的抑杀作用,并设立新肤螨灵软膏为阳性对照、生理盐水为阴性对照,进行体外抗螨活性筛选。应用健康家兔进行皮肤刺激实验和急性皮肤毒性实验。结果:丁香、枳壳、细辛、桂皮、良姜和花椒6种中药的挥发油具有较好的抑杀蠕形螨效果,对2种蠕形螨均有明显的抑杀作用,其中丁香、细辛2种中药挥发油对毛囊蠕形螨的抑杀效果优于皮脂蠕形螨(P<0.05) 。丁香挥发油对家兔完整皮肤的刺激评分为0.2,破损皮肤的刺激评分为0.3,无明显毒性。结论:丁香、枳壳等6种中药挥发油具有良好的体外抑杀蠕形螨活性,其中丁香挥发油具有较强的体外抗蠕形螨活性且具有皮肤安全性。

Volatile oil of Chinese crude drug against human demodex:effectiveness and safety

Objective:To search for effective volatile oil of Chinese crude drug for killing demodex in vitro.Methods: The demodex mites were collected by adhesive cellophane tape technique.The killing effects of different volatile oils against Df and Db were observed by microscope.Normal saline and Xinfu Manling Ointment were used as negative and positive controls,respectively.Skin irritation tests for normal and wounded skin and acute toxicity test were carried out using rabbit skin.Results: The volatile oils of 6 Chinese crude drugs,including clore,orange fruit,manchurian wildginger,cinnamon bark,rhizoma alpinae,and pricklyash peel,were highly potent in killing Df and Db in vitro; and the volatile oil of clore and manchurian wildginger had a better killing effect against Df than Db(P<0.05).The scores for irritation to normal and wounded rabbit skin were 0.2 and 0.3,respectively,and acute toxicity test showed no obvious toxicity.Conclusion: The volatile oils of the 6 mentioned Chinese crude drugs have satisfactory anti-mite activities in vitro.The volatile oil of clore shows a remarkable activity against demodex in vitro,with good skin safety.