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Measurement of insulin-like growth factor-1 and insulin-like growth factor binding protein-3 after delayed separation of whole blood samples |
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| Authors: | Hartog Hermien van der Graaf Winette T A Wesseling Jelle van der Veer Eveline Boezen H Marike |
| Institution: | aDepartment of Medical Oncology, University Medical Center Groningen, University of Groningen, Groningen, the Netherlands bDepartment of Medical Oncology, Radboud University Nijmegen Medical Center, Nijmegen, the Netherlands cDepartment of Pathology, The Netherlands Cancer Institute/Antoni van Leeuwenhoek Hospital, Amsterdam, the Netherlands dClinical Laboratories, University Medical Center Groningen, University of Groningen, Groningen, the Netherlands eDepartment of Epidemiology, University Medical Center Groningen, University of Groningen, the Netherlands |
| Abstract: | Objectives:Epidemiological studies benefit from unbiased blood specimens collected with minimal cost and effort of blood collection and storage. We evaluated the stability of IGF-1 and IGFBP-3 in whole blood samples stored at room temperature to justify delays in blood processing.Design and methods:Total IGF-1 and IGFBP-3 levels were measured in EDTA plasma (n = 12), heparin plasma (n = 12) and serum (n = 10) samples of healthy volunteers after blood processing delays up till 14 days. Stability of measured IGF-1 and IGFBP-3 levels was tested by paired t-test and a linear mixed effect model.Results:Longitudinal analysis showed that IGF-1 levels were not significantly affected by blood processing delays in EDTA tubes (p = 0.18) and IGFBP-3 levels were marginally stable (p = 0.06). In heparin plasma and serum, however, IGF-1 increased over time of delayed processing and IGFBP-3 levels tended to decrease (p < 0.01).Conclusion:Total IGF-1 and IGFBP-3 levels are stable in whole blood collected in EDTA tubes at room temperature up till 7 days, allowing a delay in blood processing to reduce costs in large multi-center studies. |
| Keywords: | IGF-1 IGFBP-3 Storage Stability Serum Plasma Whole blood |
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