磷脂酰胆碱对血管性痴呆大鼠学习记忆的影响

目的:探讨大豆磷脂酰胆碱对血管性痴呆大鼠学习记忆能力的作用。方法:大鼠随机分为假手术组、模型组和磷脂酰胆碱组,采用双侧颈总动脉结扎法(2-VO法)建成血管性痴呆大鼠模型,分别通过Morris水迷宫系统和免疫荧光方法观察各组大鼠行为学和海马CA3区脑源性神经营养因子的变化。结果:长期服用磷脂酰胆碱可明显改善血管性痴呆模型大鼠的Morris水迷宫测试成绩,同时海马CA3区BDNF阳性细胞数目明显增多,光密度值减弱。结论:长期服用磷脂酰胆碱可对抗大鼠脑缺血后所致的学习记忆的损害。

EFFECT OF PHOSPHATIDYLCHOLINE ON SPATIAL LEARNING AND MEMORY IN RATS WITH VASCULAR DEMENTIA

Objection: To investigate the preventive effects of soybean phosphatidylcholine(PC)on spatial learning and memory deficits in rats with vascular dementia(VD).Methods: The rats were divided into three groups at random: sham-operated group,model group and soybean PC group.Models of VD were established by permanent occlusion of the bilateral common carotid arteries(2-VO methods),and subsequently all rats were tested in Morris water maze for 5 d.At the end of experiment,hippocampus of each rat was dissected and the brain derived neurotrophic factor(BDNF) was tested using immunohisto-chemical method.Results: In VD rats fed with soybean PC for long time,the maze testing score was significantly improved.Immunohistochemical results showed that BDNF positive neurons in hippocampus were significantly increased and OD values reduced.Conclusion: Soybean phosphatidylcholin can prevent learning and memory impairment of cerebral blood flow in rats with VD.