儿童肾透明细胞癌的病理及其癌细胞DNA定量分析

目的：探讨儿童肾透明细胞癌病理形态特点及癌细胞DNA含量和倍体的关系。方法：对4例儿童肾透明细胞癌组织进行病理形态观察,并用图象分析仪对癌细胞DNA进行定量分析。结果：4例癌细胞胞质透明呈透明细胞且有乳头状结构,其中2例乳头状结构超过50％,3例均见钙化小体及明显出血坏死,DNA检测,DNA平均指数1．31,呈2倍体型,高2倍体型或亚4倍体型,结论：儿童肾透明细胞癌病理形态特点为癌细胞胞质透明,以乳头状结构为主,可见钙化小体,常伴出血,坏死,癌周肾小球,肾小管基本正常为特征,癌细胞DNA呈2倍体或高2倍体型或亚4倍体型。

The pathology and DNA quantitative study of renal clear cell carcinoma in children

Objective To investigate the pathological features of renal clear cell carcinoma (RCCC) in children and its relationship to the DNA content of cancer cells and DNA ploidy. Methods The pathologic morphology of 4 cases of RCCC in children were observed. Using image analysis instruments the quantity of tumor cell DNA was measured. Results The cytoplasm of the tumor cells were clear in all four cases. Papillary structures were present in these tumors, of which the papillary structure in two cases was over 50%, three cases had small calcified bodies with prominent bleeding and necrosis. Around tumor the glomeralar and tubular of kidney always is normal the average DNA index was 1.31. They presented diploidy, high diploidy or subtetraploidy. Conclusions The specific features of most RCCC in children are clear cytoplasm, most contain papillary structures, calcified bodies, often with bleeding and necrosis. Around tumor the glomeralar and tubular of kidney is always normal. The DNA contents of cancer cells present diploidy, high diploidy or subtetraploidy.