| 儿童EB病毒相关噬血细胞淋巴组织细胞增生症NK细胞表面受体和CD107a表达 |
| |
| 引用本文： | 秦强,谢正德,刘春艳,黄志卓,王亚丽,张寒,赵晓曦,申昆玲.儿童EB病毒相关噬血细胞淋巴组织细胞增生症NK细胞表面受体和CD107a表达[J].中国医学文摘：基础医学,2012(2):136-140. |
| |
| 作者姓名： | 秦强 谢正德 刘春艳 黄志卓 王亚丽 张寒 赵晓曦 申昆玲 |
| |
| 作者单位： | [1]首都医科大学附属北京儿童医院,北京市儿科研究所,儿科学国家重点学科,省部共建儿科重大疾病研究重点实验室 [2]首都医科大学附属北京儿童医院儿童血液肿瘤中心 ,北京市儿科研究所,儿科学国家重点学科,省部共建儿科重大疾病研究重点实验室 [3]首都医科大学附属北京儿童医院呼吸感染中心北京,100045 |
| |
| 基金项目： | 国家自然科学基金：81171568; 北京市自然科学基金：7113152; 中国博士后科学基金：20090460396; 北京市卫生系统高层次卫生技术人才专项经费（学科骨干培养计划）：2009-3-42 |
| |
| 摘 要： | 目的研究儿童EB病毒相关噬血细胞淋巴组织细胞增生症（EBV-HLH）NK细胞表面受体和CD107a表达,为深入了解EBV-HLH发病机制提供理论依据。方法选取2009年8月至2011年5月于北京儿童医院确诊为EBV-HLH、未接受过化学免疫治疗的住院患儿为EBV-HLH组,选取年龄、性别与EBV-HLH组1∶1匹配的健康查体儿童为正常对照组。应用流式细胞仪检测NK细胞表面受体、穿孔素及CD107a表达。予IL-2刺激后再次测定CD107a表达情况。结果 EBV-HLH组和正常对照组各8例。①两组NK细胞表面受体NKp30、NKp46、NKG2D、DNAM-1和2B4表达阳性率差异均无统计学意义,CD56＋NK细胞穿孔素表达率和CD8＋T细胞穿孔素表达率差异均无统计学意义。②两组在IL-2刺激前CD107a、CD107a/K562、CD107a/2B4/P815、CD107a/NKG2D/P815和CD107a/2B4/NKG2D/P815表达率差异均无统计学意义。③EBV-HLH组在IL-2刺激前后CD107a表达差异无统计学意义;正常对照组IL-2刺激前后CD107a、CD107a/K562和CD107a/NKG2D/P815表达率差异有统计学意义。结论 EBV-HLH患儿NK细胞对IL-2的反应出现异常,可能与EBV-HLH的发生有关。
|
| 关 键 词： | NK细胞 EB病毒 噬血细胞淋巴组织细胞增生症 儿童 病例对照研究 |
Expression of NK cell surface receptors and CD107a in Epstein-Barr virus associated hemophagocytic lymphohistiocytosis children |
| |
| Authors: | QIN Qiang XIE Zheng-de LIU Chun-yan HUANG Zhi-zhuo WANG Ya-li ZHANG Han ZHAO Xiao-xi SHEN Kun-ling |
| |
| Institution: | 1 Key Laboratory of Major Diseases in Child and National Key Discipline of Pediatrics, Capital Medical University, Ministry of Education, Beijing. Beijing Pediatric Research Institute, Beijing Children＇s Hospital, Capital Medical University, Beijing 100045, China; 2 Department of Hematology, Beijing Children＇s Hospital, Capital Medical University, Beijing 100045, China; 3 Department of Infectious Diseases, Beijing Children＇s Hospital, Capital Medical University, Beijing 100045, China) |
| |
| Abstract: | Objectives The role of NK cell function as an etiological or prognostic factor in EBV-HLH remains unclear. The present study tried to investigate the expression of NK cell surface activating receptors and CD107a in EBV-HLH patients. Methods EBV-HLH patients and age- and sex-matched normal controls were selected from August 2009 to May 2011 in Beijing Children＇s Hospital. Multicolored immunofluorescence flowcytometric analysis was performed to detect NK cellsurface activating receptors, perforin and IL-2 stimulated NK cells in peripheral blood from EBV-HLH patients and controls. Results Eight cases were enrolled in EBV-HLH group and 8 normal children were enrolled in control group. ①There was no statistical difference between EBV-HLH patients and normal controls in NK cell surface activating receptors （NKP30, NKP46, NKG2D, DNAM-1 and 2B4）, perforin or basic CD107a expression （P0.05）. ②There was no statistical difference in basic CD107a, CD107a/K562, CD107a/2B4/P815, CD107a/NKG2D/P815 or CD107a/2B4/NKG2D/P815 expression between EBV-HLH group and control group. ③Basic CD107a expression, CD107a/K562 and CD107a/NKG2D/P815 expression in normal controls significantly changed 48 hours after IL-2 stimulation, while such difference was not seen in EBV-HLH patients （P0.05）. Conclusions The present results indicated that there may be some defects in responding to IL-2 in EBV-HLH patients. |
| |
| Keywords: | NK cell Epstein-Barr virus Hemophagocytic lymphohistiocytosis Child Case-control study |
| 本文献已被 维普 等数据库收录！ |
|
