HCV RNA载量与肝脏组织损伤的关系

目的探讨丙型肝炎病毒（HCV）RNA复制水平与肝功能丙氨酸氨基转移酶（ALT）、天门冬氨酸氨基转移酶（AST）之间的相互关系。方法收集1182例疑似丙肝病人血清标本,采用荧光定量聚合酶链反应（FQ-PCR）检测HCV RNA,利用酶动力法测定ALT、AST,采用ELISA测定抗HCV抗体。数据采用SPSS16.0统计软件处理。结果病人血清样本中,HCV RNA阳性801例（64.8%）,其中抗HCV阳性766例（95.6%）;HCV RNA阴性381例（32.2%）,其中抗HCV阳性100例（26.2%）。HCV RNA阳性样本中同时抗-HCV阳性者ALT,AST异常率分别为59.0%和60.8%;抗-HCV阴性者为31.4%和34.2%。HCV RNA阴性样本中同时抗-HCV阳性者ALT,AST异常率分别为24.0%和15.0%,抗-HCV阴性者为13.9%和12.1%。HCV RNA含量与ALT、AST水平明显成正相关,r=0.62,P〈0.05。结论 HCV RNA含量与肝组织损伤程度成正相关。

Investigation for the Relationship between HCV-RNA level and Hepatic Insufficiency

Objective To investigate the relationship between HCVRNA load and levels of ALT and AST.Methods 1182 samples form suspected HCV infected patients were collected for analysis.The level of HCV RNA in the serum were detected by FQ-PCR,and ALT,AST levels were detected by enzymatic kinetic assay.Anti-HCV was detected by ELISA.The data were analyzed by SPSS.Results For all the patients,801 of them were HCV-RNA positive,731 of whom were anti-HCV positive.381 of the patients were HCV-RNA negative and 100 of whom were anti-HCV positive.The abnormal rate of ALS and AST of patients with both HCV-RNA and anti-HCV positive was 59.0% and 60.8% respectively;and that of patients with HCV-RNA positive and anti-HCV negative was 31.4% and 34.2% respectively.On the other hand,That of patients with HCV-RNA negative and anti-HCV postive was 24.0% and 15.0% respectively;and that of patients with HCV-RNA negative and anti-HCV negative was 13.9% and 12.1%.ALT and AST level varied directly with HCV-RNA level,r=0.62,P0.05.Conclusion HCV-RNA level showed directly proportional with hepatic insufficiency.