| 实时荧光PCR在检测MRSA引起血流感染中的研究 |
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| 引用本文： | 张志明,朱建伟,李建平.实时荧光PCR在检测MRSA引起血流感染中的研究[J].中国实验诊断学,2011,15(3):384-386. |
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| 作者姓名： | 张志明 朱建伟 李建平 |
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| 作者单位： | 西安市中心医院,检验科,陕西,西安,710003 |
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| 基金项目： | 陕西省卫生厅科学研究基金 |
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| 摘 要： | 目的建立检测引起血流感染中耐甲氧西林金黄色葡萄球菌（MRSA）mecA基因的实时荧光PCR法。方法设计mecA引物,优化荧光实时PCR实验条件,并用该法检测临床分离的78株金黄色葡萄球菌引起血流感染标本,其结果与头孢西丁纸片扩散法的结果进行比较。结果 78株金黄色葡萄球菌中,荧光实时PCR法检测出mecA基因阳性38株、阴性40株;头孢西丁纸片扩散法检测出MRSA 27株、MSSA 51株,经配对卡方检验,荧光实时PCR法检出率显著高于头孢西丁纸片扩散法（P〈0.01）。结论荧光实时PCR法能敏感、特异检测MRSA引起的血流感染,比一般MRSA表型检测更加简便、快速。
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| 关 键 词： | 耐甲氧西林金黄色葡萄球菌 mecA基因 血流感染 |
Research of Real-time fluorescence PCR in the detection of bloodstream infections caused by MRSA |
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| ZHANG Zhi-ming,ZHU Jian-wei,LI Jian-ping.Research of Real-time fluorescence PCR in the detection of bloodstream infections caused by MRSA[J].Chinese Journal of Laboratory Diagnosis,2011,15(3):384-386. |
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| Authors: | ZHANG Zhi-ming ZHU Jian-wei LI Jian-ping |
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| Institution: | ZHANG Zhi-ming,ZHU Jian-wei,LI Jian-ping(Department of Clinical Laboratory,Xi'an Central Hospital,Xi'an 710003,China) |
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| Abstract: | Objective To establish the detection of bloodstream infections caused by methicillin-resistant Staphylococcus aureus(MRSA) mecA gene by real-time PCR.Methods Designed mecA primers and optimized experimental conditions for fluorescence real-time PCR,and used this method to detect the 78 clinical isolates of Staphylococcus aureus,compared the results with the Fox disk diffusion method.Results Among 78 strains of Staphylococcus aureus by real-time PCR method to detect fluorescence mecA gene positive 38 strains... |
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| Keywords: | methicillin-resistant Staphylococcus aureus mecA gene bloodstream infections |
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