硇砂提取物治疗小鼠Lewis肺癌的效果初步评价

目的评价硇砂提取物治疗小鼠Lewis肺癌的效果及对免疫系统的毒性.方法采用MTT法及流式细胞仪分别检测Lewis肺癌细胞的增殖和细胞周期,采用小鼠Lewis肺癌皮下移植肿瘤模型观察硇砂提取物的抗肿瘤作用,通过小鼠碳粒廓清和迟发性变态反应考察硇砂提取物对免疫系统的毒性.结果硇砂提取物可剂量依赖性抑制Lewis肺癌细胞的增殖,使细胞周期停止于S期.硇砂提取物水溶液连续瘤内给药8 d对小鼠Lewis肺癌皮下肿瘤的抑瘤率为46.7%,但同时灌胃给药对肿瘤的抑瘤率仅为15.7%.硇砂提取物水溶液皮下注射或灌胃给药对小鼠碳粒廓清能力及迟发性变态反应均无明显影响.结论硇砂提取物的抗肿瘤作用与给药途径有关,其对机体自身的免疫功能无明显影响.

The Primary Evaluation of Sal-Ammoniac Extract on the Therapy Action in Mice Bearing Lewis Lung Cancer

OBJECTIVE: To evaluate the therapy action of Sal-Ammoniac extract on Lewis lung cancer and its toxicity to immunity. METHODS: The proliferation and cell cycle of Lewis lung cancer cells were determined by MTT assay and flow cytometry respectively. The antitumor effect of Sal-Ammoniac extract was observed by tumor injected subcutaneously in mice and its toxicity to immunity was examined by clearance rate of charcoal particles and delayed type hypersensitivity. RESULTS: Sal-Ammoniac extract could inhibit the proliferation of Lewis lung cancer cells with S cell cycle arrest in a dose-dependent manner in vitro. Sal-Ammoniac extract solution injected in tumor for eight days had 46.7% inhibition on Lewis lung cancer, if taken orally had only 15.7% inhibition on Lewis lung cancer in mice. Sal-Ammoniac extract solution injected subcutaneously or taken orally had no effect on the clearance rate of charcoal particles and delayed type hypersensitivity in mice. CONCLUSION: The antitumor action of Sal-Ammoniac extract has relation to its recipe and has no influence on immunity.