芪丹颗粒剂对大鼠肺间质纤维化时TGF-β和TNF-α表达的影响

为探讨芪丹颗粒剂对平阳霉素所致大鼠肺间质纤维化的治疗作用及机制 ,采用平阳霉素〔5mg/ (kg·d)〕气管内注入建立大鼠肺间质纤维化模型 ,观察芪丹组〔2 50mg/ (kg·d)〕与氢化可的松腹腔注射组〔2 5mg/ (kg·d)〕治疗后肺组织病理学、胶原蛋白沉积、TGF β和TNF α的表达。发现 :芪丹组肺泡炎及肺纤维化程度、胶原纤维沉积均明显低于模型组和激素组。正常组TGF β、TNF α弱表达 ,模型组强表达 ,芪丹组的表达显著少于模型组和激素组 (P <0 .0 1)。提示 :芪丹颗粒剂可明显减轻大鼠肺泡炎和肺纤维化的程度 ,而且明显优于激素组

Therapeutic Effects of Qidan Granule and Expressions of TGF-β and TNF-αon Rats with BlemycinA5 Induced Pulmonary Fibrosis

The aim was to evaluate the therapeutic effects and mechanisms of Qidan granule on blemycinA₅-induced pulmonary interstitial fibrosis (PIF)in rats. Altogether 40 rats we re divided into normal group, model group, Qidan group and Hydrocortisone group. Rat pulmonary f ibrosis was induced by blemycinA₅. They were treated with Qidan granule and Hy dr ocortisone respectively. The pathological changes and collagen protein dispositi on were observed by hematoxylin and eosin(HE) staining and Van Gieson(V.G)staini ng, and the expression of transforming growth factorβ(TGF-β)and tumor necrosi s factor α(TNF-α) proteins were measured by immunohistochemical technique. Af ter treatment, the pulmonary alveolitis and fibrosis were alleviated remarkable in the pathological section and collagen protein disposition in Qidan granule gr oup compared with those in the model group and hydrocortisone group (P<0.01) . The expression of TGF-β and TNF-αprotein were higher in Qidan granule grou p than those in normal group and were significantly less than those in the model group and in hydrocortisone group (P<0.01). Our studies suggest that Qidan granule would ameliorate the pulmonary alveolitis and fibrosis. TGF-β and TNF -α might play an important role in the development of alveolitis and fibrosis in rats.