高效液相色谱法测定盐酸左旋多巴异戊酯的含量

目的建立测定盐酸左旋多巴异戊酯含量的高效液相色谱法。方法色谱柱采用UltimateTMC18柱（250mm×4．6mm5μm），流动相为0．05mol／L磷酸二氢钾（磷酸调pH=3．5）-乙腈（70：30），流速为1．0mL／rain，检测波长为280mm，进样量为20μL，柱温为30℃。结果盐酸左旋多巴异戊酯质量浓度在16．1—322．0μg／mL范围内与峰面积线性关系良好（r=0．9999），平均回收率为98．41％，RSD为1．85％（n=6）。结论该方法准确、简便，精密度高、专属性强，可用于盐酸左旋多巴异戊酯的含量测定。

Determination of L- Dopa Isopentyl Ester Hydrochloride by HPLC

Objective To establish a high performance liquid chromatography（HPLC） method for the content determination of levodopa isopentyl ester hydroehloride. Methods The Ultimate C18 column （250mm×4.6mm,5μm） was used and the mobile phase was acetonitrile was 0.05 mol/L potassium dihydrogen phosphate （adjusting pH 3.5 with phosphoric acid, 70 ： 30）, the flow rate was 1.0 mL/miu. The detection wavelength was 280 nm. The column temperature was 30 ℃ and the injection volume was 20μL. Results All the related substances gained a completely chromatographic separation with the principal peak. A good linear relationship was observed in the concentration range of 16.1-322.0 μg/mL, with the correlation coefficient of 0.999 9（n=6）. The average recovery rate was 98.41% and RSD was 1. 85% （n =6）. Conclusion The method is proved to be accurate and sensitive with high precision and strong specificity, which can be applicable for the content assay of levodopa isopentyl ester hydrochloride.