| 大孔树脂分离纯化独一味总黄酮的工艺研究 |
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| 引用本文： | 王永,王世华,安军永,王超,秦拢,王猛,李云鹏,李向军. 大孔树脂分离纯化独一味总黄酮的工艺研究[J]. 中国药业, 2012, 21(5): 25-27 |
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| 作者姓名： | 王永 王世华 安军永 王超 秦拢 王猛 李云鹏 李向军 |
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| 作者单位： | 石家庄以岭药业股份有限公司,河北,石家庄,050035 |
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| 摘 要： | 目的 研究大孔树脂分离纯化独一味总黄酮的工艺条件与技术参数.方法 采用高效液相色谱法测定山栀苷甲酯和8-0-乙酰山栀苷甲酯的含量,紫外分光光度法测定独一味中总黄酮的含量.结果 D-101树脂对独一味总黄酮的富集效果最好.吸附条件为药液质量浓度1 g/mL,原液室温上样,吸附流速3 BV/h,洗脱剂为70%乙醇,洗脱流速5 BV/h,洗脱剂用量为10 Bv.结论 试验工艺稳定、可行.
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| 关 键 词： | 大孔树脂 独一味 总黄酮 分离 纯化 |
Separation and Purification of Lamiophlomis Rotata Total Flavonoids |
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| Wang Yong , Wang Shihua , An Junyong , Wang Chao , Qian Long , Wang Meng , Li Yunpeng , Li Xiangjun. Separation and Purification of Lamiophlomis Rotata Total Flavonoids[J]. China Pharmaceuticals, 2012, 21(5): 25-27 |
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| Authors: | Wang Yong Wang Shihua An Junyong Wang Chao Qian Long Wang Meng Li Yunpeng Li Xiangjun |
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| Affiliation: | Wang Yong, Wang Shihua,An Junyong, Wang Chao, Qian Long, Wang Meng, Li Yunpeng, Li Xiangjan (Shifiazhuang Yiling Pharmaceutical Co., Ltd., Shijiazhuang, Hebei, China 050035) |
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| Abstract: | Objective To research the separation and purification of Lamiophlomis rotata total flavonoids conditions and technical parameters. Methods Determination of gardenia glycoside 8- O- methyl and acetyl methyl glucoside by HPLC, single blind UV spec-trophotometry of total flavonoids in the investigated macroporous resin purification process. Results D- 101 resin for the enrichment of total flavonoids Lamiophlomis rotata best. Adsorption conditions were liquid concentration l g/mL （the equivalent of raw medicinal herbs） ,the sample liquid at room temperature,the adsorption velocity 3 BV/h（BV muhiple of the column bed volume）,70% ethanol eluant,elution flow rate 5 BV/h,eluting agent is 10 BV. Conclusion The process is stable and usable. |
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| Keywords: | D - 101 macropere resin Lamiophlomis rotata total flavonoids separation purification |
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