随机引物聚合酶链反应对耐甲氧西林金黄色葡萄球菌基因分型的研究

目的建立耐甲氧西林金黄色葡萄球菌（ＭＲＳＡ）随机引物聚合酶链反应（ＡＰ－ＰＣＲ）基因分型的方法用于；临床流行病学调查。方法优化ＡＰ－ＰＣＲ方法并与抗生素敏感谱分型法比较，评价ＡＰ－ＦＣＲ基因分型法的可靠性与分辨力；并对４９株ＭＲＳＡ进行ＡＰ－ＲＣＲ基因分型。结果４９株医院感染的ＭＲＳＡ可分为１０个ＡＰ－ＰＣＲ谱型，主要谱型为 Ａ３型，分型率 １００％；其中 ４１株 ＭＲＳＡ为流行病学相关株。结论 ＡＰ－ＰＣＲ基因分型是一种经济、快捷、可靠的分型方法；是在分子水平上对微生物感染进行病原学、发病机理及流行病学研究较理想的工具。

Arbitrarily primed polymerase chain reaction for genotyping of meticillin resistant Staphylococcus aureus strains

OBJECTIVE Genotyping by arbitrarily primed polymerase chain reaction(AP-PCR) wes established and used for in- vestigation of clinical epidemiology. METHODS AP-PCR was optimized and compared with antibiotic susceptibility typing for evaluating reproducibility and discriminatory abilities of AP - PCR typing; and was used to type 4 9 strains MRSA. RESULTS Forty-nine strains of Staphylococcus aureus nosocomial infections were divided into 10 AP-PCR profiles, typing rate was 100 percent; 41 strains MRSA were epidemiological related strains. CONCLUSIONS Genotyping by AP-PCR is very cheap, rapid and reliable. It is an ideal tool for study of etiology, pathogenesis and epidemiology at molecular level in microorganism infection.