多指标正交试验法优选加味败藤颗粒的提取工艺

目的:优选加味败藤颗粒的水提取工艺。方法:以盐酸小檗碱、芍药苷质量及含固量的综合评分为指标,通过L9(3)4正交试验考察加水量、提取时间和煎煮次数对加味败藤颗粒水提取工艺的影响。采用HPLC-DAD测定盐酸小檗碱和芍药苷含量,流动相10 mmol·L-1磷酸二氢钾水溶液(A)-乙腈(B)梯度洗脱(0~9 min,19.5%B;9~10 min,19.5%~45%B;10~20min,45%B),检测波长分别为347,231 nm。结果:最佳提取工艺为加10倍量水煎煮3次,每次1 h;含固量23.69%,盐酸小檗碱、芍药苷质量依次为0.092 5,0.333 7 g。结论:优选的工艺合理可行,重复性好,为加味败藤颗粒的工业化生产提供参考。

Optimization of Extracting Technology for Jiawei Baiteng Granules by Multi-index Orthogonal Test

Objective: To optimize water extracting technology of Jiawei Baiteng granules. Method: Withcomposite score of contents of berberine hydrochloride, paeoniflorin and solid-containing as index, orthogonal test was adopted to optimize extraction process by taking the amount of water, extraction time and boiling times as factors.Contents of berberine hydrochloride and paeoniflorin were determined by HPLC coupled with DAD detector, mobile phase consisted of 10 mmol· L^-1 potassium dihydrogen phosphate solution (A)-acetonitrile(B) with gradient elution program of(0-9 min, 19.5% B;9-10 min, 19.5-45% B;10-25 min, 45% B), detection wavelengths were set at 347 nm and 231 nm, respectively. Result: Optimal extracting technology was as follows:decocted 3 times with 10 times the amount of water for 1 h at each time;under these conditions, contents of berberine hydrochloride, paeoniflorin and solid-containing were 0.092 5 g, 0.333 7 g and 23.69%, respectively. Conclusion: This optimizeprocesswas reasonable, feasible and reproducible, which could guide industrial production of Jiawei Baiteng granules.