虎杖总鞣质的提取工艺优选及吸湿性考察

目的:优选虎杖总鞣质的提取工艺并考察其吸湿性。方法:采用络合滴定法测定总鞣质含量。以总鞣质提取量为指标,通过单因素试验确定提取温度,利用正交试验考察丙酮体积分数、提取次数、料液比对总鞣质提取工艺的影响。将总鞣质粉末置于恒温恒压恒湿环境中,通过每隔一段时间检测的质量变化以考察该成分的吸湿特性。结果:最佳提取工艺为加5倍量30%丙酮超声提取3次,每次30 min;总鞣质平均提取量102.8 mg·g-1,RSD 1.25%。虎杖总鞣质的临界相对湿度约50%,在恒温恒压恒湿环境中24 h后基本达吸湿平衡。结论:优选的提取工艺稳定可行,虎杖总鞣质的吸湿性较强。

Optimization of Extraction Technology of Total Tannins from Polygoni Cuspidati Rhizoma et Radix and Investigation of Its Hygroscopicity

Objective: To optimize extraction process of total tannins in Polygoni Cuspidati Rhizoma et Radix and investigate its hygroscopicity. Method: The content of total tannins was determined by complexometric titration. Taking extracting amount of total tannins as index, extraction temperature was selected by single factor test, orthogonal test was adopted to optimize extraction process with acetone concentration, extracting times and solid-liquid ratio as factors.Total tannins powder was placed in environment with constant temperature, humidity and pressure, weight change was measured periodically to investigate its hygroscopic properties. Result: Optimum extraction process was as following:extracted thrice with five times the amount of 30% acetone for 30 min each time;average extracting amount of total tannins was 102.8 mg· g^-1 with RSD of 1.25%.Critical relative humidity of total tannins in Polygoni Cuspidati Rhizoma et Radix was about 50%, it reached hygroscopic balance after 24 h in the environment with constant temperature, humidity and pressure. Conclusion: Optimized extraction process was stable and feasible, total tannins in Polygoni Cuspidati Rhizoma et Radix had strong hygroscopicity.