葛根-麻黄药对不同比例配伍对葛根素溶出量的影响

目的:探讨葛根-麻黄药对不同比例配伍对葛根素溶出量的影响。方法:采用HPLC测定葛根素含量,流动相甲醇-水(25∶75),检测波长250 nm,建立葛根-麻黄药对中葛根素的含量测定方法。考察葛根-麻黄药对不同比例(3∶1,2∶1,4∶3,1∶1,1∶2,1∶3)配伍对葛根素溶出量的影响。结果:建立的含量测定方法稳定可行,平均加样回收率99.67%,RSD 2.2%。葛根-麻黄按4∶3配伍时葛根素溶出量最多(2.408 g·L-1),与葛根水煎液(2.455 g·L-1)最接近,而葛根-麻黄按2∶1配伍时溶出量最少(2.254 g·L-1)。结论:不同配伍比例的麻黄对葛根中葛根素的溶出量具有一定影响。

Effects of Different Proportions of Couplet Medicines of Puerariae Lobatae Radix and Ephedrae Herba on Dissolution of Puerarin

Objective: To investigate influence of different proportions between Puerariae Lobatae Radix and Ephedrae Herba on dissolution of puerarin. Method: HPLC was used to determine the content of puerarin with mobile phase of methanol-water (25:75) and detection wavelength at 250 nm, methodological study of puerarin from couplet medicines of Puerariae Lobatae Radix and Ephedrae Herba was established.Effects of couplet medicines of Puerariae Lobatae Radix and Ephedrae Herba with different proportions (3:1, 2:1, 4:3, 1:1, 1:2, 1:3) on dissolution of puerarin were investigated. Result: This established determination was stable and feasbile with average recovery of 99.67% (RSD 2.2%).The maximum dissolution (2.408 g· L^-1) of puerarin was achieved when proportions of Puerariae Lobatae Radix and Ephedrae Herba was 4:3, it was closed to Puerariae Lobatae Radix decoction (2.455 g· L^-1), the minimum dissolution (2.254 g· L^-1) of puerarin was achieved when proportions of Puerariae Lobatae Radix and Ephedrae Herba was 2:1. Conclusion: Different proportions of Puerariae Lobatae Radix and Ephedrae Herba had some influence on dissolution of puerarin.