基质金属蛋白酶-7和碱性成纤维细胞生长因子在非小细胞肺癌中的表达意义及相关性

目的检测基质金属蛋白酶(MMP)-7和碱性成纤维细胞生长因子(bFGF)在非小细胞肺癌组织中的表达情况,并分析其与非小细胞肺癌的病理分化程度、手术-病理分期、淋巴结转移的相关性,探讨MMP-7和bFGF在非小细胞肺癌的发生、浸润及转移过程中的作用。方法采用免疫组织化学S-P法检测原发性非小细胞肺癌石蜡包埋组织80例、正常肺组织40例中MMP-7和bFGF蛋白的表达水平。结果 1非小细胞肺癌组MMP7和bFGF阳性率均高于正常肺组织组(P=0.001)。2非小细胞肺癌组TNM分期中Ⅰ^Ⅱ期合并后和ⅢⅡ期合并后和Ⅲ^Ⅳ期合并后MMP-7和bFGF的阳性率比较,差异有统计学意义(P均=0.000);低分化癌的MMP-7和bFGF的阳性率与高、中分化癌相比,差异亦有统计学意义(P=0.001,P=0.013);淋巴结转移组MMP-7和bFGF的表达与无淋巴结转移组相比差异也有统计学意义(P=0.003,P=0.000);3非小细胞肺癌中MMP-7和bFGF的表达具有相关性(r=0.377,P=0.001)。结论 MMP-7与bFGF可能参与了非小细胞肺癌的发生和演进的过程;MMP-7与bFGF在非小细胞肺癌的发生、浸润和转移的过程中可能具有协同作用;MMP-7与bFGF蛋白的联合检测,可能成为判断非小细胞肺癌发生发展和评价预后的有效参考指标之一,对于非小细胞肺癌的早期预测可能具有重要的临床意义。

The expression and correlat ion of matrix metalloproteinase and basic fibroblast growth factor in non-small cell lung cancer

Objective To investigate the expressive significance of matrix metalloproteinase （MMP）-7 and basic fibroblast growth factor（bFGF） in non-small cell lung cancer（NSCLC） and explore the correlation between the expression and tumor differentiation, staging or lymph node metastasis,and to elucidate the role of MMP-7 and bFGF in tumor occurrence, invasion and metastasis of NSCLC. Methods The expression of MMP-7 and bFGF in 80 cases of NSCLC and 40 cases of normal lung tissue were detected immunohistochemically by S-P method. Results ① The positive rate of MMP-7 and bFGF was higher in NSCLC group than that in normal lung tissue（P=0.001）.②In NSCLC, the expression of MMP-7 and bFGF in stagingⅢ-Ⅳgroup was significantly different from the stagingⅠ-Ⅱgroup（P=0.001）. The expression of MMP-7 and bFGF in G3 group of NSCLC was also significantly different from G1-G2 group （P=0.001, P=0.013）. The expression of MMP-7 and bFGF in group with lymph node metastasis was also significantly different from that in group without lymph node metastasis （P=0.003, P=0.001）. ③ The expression of MMP-7 was positively correlated to bFGF in NSCLC （r=0.377, P=0.001）. Conclusion The results indicate that both MMP-7 and bFGF may participate in the process of occurrance and progression in NSCLC, and may have synergistic effet during this process. Combined detection of MMP-7 and bFGF may be a effective parameter in evaluate occurrance, progression and prognosis of NSCLC, and may also have important clinical significance in early prediction of NSCLC.