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PCR检测淋球菌的临床应用价值
引用本文:李杰,张启英.PCR检测淋球菌的临床应用价值[J].蚌埠医学院学报,1996,21(1):61-62.
作者姓名:李杰  张启英
作者单位:Department of Clinical Laboratory,Affiliated Hospital of Bengbu Medical College,Anhui 23 3004
摘    要:本文应用聚合酶链反应技术,快速检测淋病奈瑟菌,对高度疑似淋病的72例检测中,49例为阳性结果,同时作涂片配对检测,仅27例为阳性结果(P〈0.005);其中,42例用PCR法和培养法配对检查,PCR法阳性为28例,培养法阳性为12例(P〈0.005)。109株淋球菌药敏试验结果表明,耐青霉素类药物菌株仅为26.6%,仍不失为治疗淋病的首选药物。

关 键 词:聚合酶链反应  淋病奈瑟菌  淋病  药敏试验

Evaluation of PCR detection of Neisseria gonorrhoeae
Li Jie,Zhang Qiying, Guo Cai,Liu Jun.Evaluation of PCR detection of Neisseria gonorrhoeae[J].Journal of Bengbu Medical College,1996,21(1):61-62.
Authors:Li Jie  Zhang Qiying  Guo Cai  Liu Jun
Abstract:In this article,rapid detection of Neisseria gonorrhoeae was evaiuated by compared with smear examina-tion and cultivation, Among 72 cases strongly suspected of having N. gonorrhoeae,49 cases were positive in PCR,and only 27 cases were positive in smear examination,Among 42 cases examined 28 cases were positive in PCR and12 positive by cultivation. The susceptibility test of 109 strains of N. gonorrhoeae reverled that only 26.6% were re-sistant to penicillins.So penicillins is still the drug of choice in treatment of gonorrhoeae.
Keywords:polymerase chain reaction  Neisseria gonorrhoeae  gonorrhea  susceptibility testing  
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