肺癌间质纤维母细胞的增殖活性及其意义

背景与目的肿瘤细胞与间质细胞的相互作用在肿瘤的侵袭和转移中起着重要作用。本研究的目的是观察肺癌间质纤维母细胞的增殖活性,探讨纤维母细胞增殖活性与肺癌转移的关系。方法采用免疫组织化学方法检测94例手术切除肺癌组织中纤维母细胞的增殖活性。结果在所有肺癌病例中,无论肿瘤大小,肿瘤中心区瘤细胞PCNA标记指数(LI)均明显低于周围区瘤细胞(P<0.05),而肿瘤中心区纤维母细胞PCNALI显著高于周围区纤维母细胞(P<0.05)。癌旁肺组织中纤维母细胞PCNALI明显低于肿瘤中心区和周围区纤维母细胞(P<0.05)。中心区和周围区纤维母细胞增殖活性与肿瘤细胞的增殖活性明显相关。有淋巴结转移组肿瘤中心区纤维母细胞PCNALI(10.4±6.7)显著高于无转移组(6.5±5.9)(P<0.05),有转移组肿瘤周围区纤维母细胞PCNALI(7.2±6.1)也显著高于无转移组(5.6±4.7)(P<0.05)。结论高度增生瘤细胞和高度增生纤维母细胞在肿瘤组织中的分布有所不同,周围区肿瘤细胞代表瘤细胞的增殖活性,中心区纤维母细胞代表间质纤维母细胞的增殖活性。

Proliferative activity of intratumoral fibroblasts in lung cancer and its implications

Background and objective The interaction between tumor cells and stroma cells plays an important role in the invasion and metastasis of tumor. The purpose of this study is to evaluate the proliferative activity of fibroblasts in lung cancer, and to explore its correlation with tumor biologic behavior. Methods Ninety-four cases of surgically resected specimens of lung cancer were collected. The proliferative activity of fibroblasts was immunohistochemically evaluated with the mouse monoclonal antibody against proliferating cell nuclear antigen (PCNA). Results The tumor cells in the outer area had significantly higher PCNA label index (LI) than those in the inner area, however, the PCNA LI of fibroblasts in the inner area was significantly higher than those in the outer area regardless of tumor size. The PCNA LI of fibroblasts in the paracancerous tissues was significantly lower than that of fibroblasts in the inner or outer area of tumor. There was a significant correlation between the PCNA LI of fibroblasts and that of tumor cells in the inner or outer area of tumor. The PCNA LI of fibroblasts in either inner or outer area showed a significant correlation with lymph node metastasis of lung cancer. Conclusion The results demonstrate that there is a discrepancy in the distribution of highly proliferative tumor cells and highly proliferative fibroblasts in lung cancer. The proliferative activity of tumor cells should be assessed in tumor cells in the outer area, and proliferative activity of fibroblasts should be assessed in fibroblasts in the inner area.