绵萆薢对去卵巢大鼠股骨生物力学及骨形态计量学的影响

目的探讨绵萆薢水煎液对雌性大鼠去卵巢后股骨生物力学及骨计量学指标的影响.方法雌性未孕大鼠随机分为假手术组、去卵巢组、绵萆薢高(4 g/kg·d)、中(2 g/kg·d)、低(1 g/kg·d)剂量组,每组均为5只.连续给药12周后,三点弯曲法测量股骨生物力学指标,并分别进行HE染色、Von Kossa染色及四环素荧光标记观察,以骨计量学方法分别测定骨小梁体积百分比TBV(%)、骨小梁类骨质表面TOS(%)、类骨质宽度MOSW(μm)、四环素单标表面MdS(%)、四环素双标表面DMds(%)、双/单标表面比DMds/MdS(%)、骨形成表面百分比TFS(%)、骨吸收表面百分比TRS(%)、骨矿化沉积率MAR(μm/d)、骨矿化延迟时间MLT(d).结果大鼠去卵巢3个月后股骨的最大载荷、挠度、最大应变分别为125.78±15.48(N)、1.87±0.22(mm)和9.34±1.10(%),均明显低于假手术组(P＜0.05,P＜0.01);各剂量绵萆薢可以不同程度地减少其最大载荷、最大应力的降低.去卵巢3个月后大鼠股骨骨体积较假手术组明显降低(P＜0.01),四环素单标表面、双标表面、双单标比及类骨质表面、类骨质宽度、破骨表面、矿化率均明显增加(P＜0.01);绵萆薢中、高剂量组的骨体积均明显高于模型组(P＜0.05),四环素单标表面、双标表面、双/单标比、骨吸收表面、类骨质表面、类骨质宽度、矿化沉积率均明显低于模型组(P＜0.05),骨矿化延迟时间稍短于模型组(P＞0.05).结论绵萆薢在一定程度上可以增加去卵巢大鼠股骨的骨小梁体积,降低骨转换率,改善部分生物力学指标.

Effects of Dioscorea septemloba on the Biomechanical and Bone Histomorphometric Parameters of Femur in the Ovariectomized Rats

OBJECTIVE: To investigate the effects of the decoction of Rhizoma Dioscoreae septemlobae (RD) on the biomechanical and bone histomorphometric parameters of femur in the ovariectomized rats. METHODS: 25 female Wistar rats without pregnancy and deliver were divided into 5 groups randomly: sham (sham-operation), ovariectomy (OVX), OVX + high dosage RD (4 g/kg x d), OVX + middle dosage RD (2 g/kg x d) and OVX + low dosage RD (1 g/kg x d), n = 5 in every group. After intragastric administration 12-week period continuously, the biomechanical and the bone histomorphometric parameters of the femur of the rats in every group were determined, respectively, including percentage of trabecular bone volume (TBV%), percentage of trabecular osteoid (TOS%), mean osteoid width (MOSW), percentage of mineralizing surface (MdS%), percentage of double mineralizing surface (DMds%), percentage of DMds/MdS (%), percentage of travecular formation surface (TFS%), percentage of trabecular resorption surface (TRS%), mineral apposition rate (MAR), mineralizing lag time (MLT). RESULTS: The maximum loading, deflection, the maximum strain of the femur in the OVX group was 125.78 +/- 15.48 (N), 1.87 +/- 0.22 (mm), 9.34 +/- 1.10 (%), it was significantly lower than that in the sham group ( P< 0.05, P < 0.01), respectively. The maximum loading and maximum stress was increased in different extent in the every dose group of OVX + RD, respectively. TBV% of femur was significantily lower in the OVX group than that in the sham group (P < 0.01). The MdS%, DMds%, DMds/MdS (%), TOS%, MOSW, TRS%, MAR was significantly higher in the OVX group than that in the sham group, respectively (P < 0.01). In the RA high and middle dosage group, the TBV% of femur was significantly higher than that in the model group (P < 0.05), and the MdS%, DMds%, DMds/MdS (%), TRS%, TOS%, MOSW, MAR was significantly lower than that in the model group, respectively (P < 0.05), and MLT was decurtated slightly (P > 0.05). CONCLUSION: The decoction of RD can decline the bone turnover and the loss of bone of femur in the ovariectomized rats.