Multiplex real-time RT-PCR for detecting chikungunya virus and dengue virus |
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| Authors: | Pongsiri Piyathida Praianantathavorn Kesmanee Theamboonlers Apiradee Payungporn Sunchai Poovorawan Yong |
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| Institution: | Center of Excellence in Clinical Virology, Department of Pediatrics, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand. |
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| Abstract: | ObjectiveTo develop diagnostic test for detection chikungunya virus (CHIKV and Dengue virus (DENV) infection.MethodsWe have performed a rapid, accurate laboratory confirmative method to simultaneously detect, quantify and differentiate CHIKV and DENV infection by single-step multiplex real-time RT-PCR.ResultsThe assay's sensitivity was 97.65%, specificity was 92.59% and accuracy was 95.82% when compared to conventional RT-PCR. Additionally, there was no cross-reaction between CHIKV, DENV, Japanese encephalitis virus, hepatitis C, hepatitis A or hepatitis E virus.ConclusionsThis rapid and reliable assay provides a means for simultaneous early diagnosis of CHIKV and DENV in a single-step reaction. |
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