蛇床子素对新生大鼠颅盖骨成骨细胞的作用

目的：研究蛇床子素（ｏｓｔｈｌｅ）在体外对新生大鼠颅盖骨成骨细胞增殖及成骨作用的影响。方法：酶消化法分离培养新生大鼠颅盖骨成骨细胞；改良Ｇｏｍｏｒｉ钙钴法染色鉴定，＾３Ｈ－胸腺嘧啶和＾３Ｈ－脯氨酸掺入法分别测定细胞增殖和胶原合成；磷酸苯二钠法测定细胞内骨碱性磷酸酶（ＡＬＰ）活性；并以抗骨质疏松症药物依普黄酮（ｉｐｒｉｆｌａｖｏｎｅ，ＩＰ）作为阳性对照药物。结果：蛇床子素对新生大鼠颅盖骨成骨细胞的增殖、ＡＬＰ的活性和胶原合成都有促进作用。其对成骨细胞的增殖作用比ＩＰ强，但对胶原合成的促进作用弱于ＩＰ。结论：蛇床子素通过增加成骨细胞数量、促进细胞胶原蛋白及ＡＬＰ的合成而促进成骨作用。

Effect of osthole on osteoblasts in neonatal rat calvarial cultures

Objective: To study the effects of osthole on proliferation and differentiation of osteoblasts in neonatal rat calvarial cultures in vitro . Methods: Osteoblasts were isolated from neonatal rat calvaria through trypsin and collagenase digestion. Quality of the cells was assessed by special ALP histochemistry. The proliferation and collagen synthesis of osteoblasts were assayed by 3H TdR and 3H proline incorporation. The activity of ALP was measured by p nitrophenyl sodium phosphate assay. Ipriflavone (IP) was used as positive control drug. Results: Osthole significantly promoted osteoblast proliferation, alkaline phosphatase activity and collagen synthesis. The effect of osthole was more potent than IP in bone cell proliferation, but was weaker than IP in collagen synthesis. Conclusion: Osthole has osteoblastic effect by increasing osteoblast proliferation, ALP activity and collagen synthesis. [