青藤碱对肿瘤坏死因子-α诱导人脐静脉内皮细胞VCAM-1表达影响初步研究

目的 进一步研究青藤碱(SIN)对肿瘤坏死因子(TNF)-α诱导脐静脉内皮细胞(HUVECs)血管细胞黏附分子(VCAM)-1表达的影响.方法 从新鲜脐带中分离培养HUVECs.用TNF-α诱导HUVECs表达VCAM-1,实验组加入不同浓度的SIN(0.25、0.0和1.0 mol/L)或地塞米松(1.0×106mol/L)分别进行或联合进行干预,用流式细胞仪检测细胞表面VCAM-1表达.结果 与TNF-α刺激组相比,SIN干预组细胞表面VCAM-1表达下降,抑制作用以1.0 mol/L最为显著.联合地塞米松(1.0x106 mol/L)进行干预,可加强SIN对TNF-α诱导的VCAM-1表达的抑制作用.结论 SIN可抑制TNF-α诱导的脐静脉内皮细胞VCAM-1的表达.青藤碱与地塞米松对TNF-α诱导的VCAM-1表达的抑制有协同作用.

Effect of sinomenine on the expression of TNF-α-induced vascular cell adhesion molecule 1 on human umbilical vein endothelial cells

Objective To further investigate the effect of sinomenine (SIN) on TNF-α-induced VCAM-1 expression in human umbilical vein endothelial cells (HUVECs). Methods HUVECs were isolated from freshly collected umbilical cords. Positive control samples were stimulated with TNF-α, but free of SIN. Negative control samples were treated in the same way, but without TNF-α and SIN. Experimental samples were co-cultured with TNF-α and SIN at various concentrations (0.25, 0.5, and 1.0 mol/L), or TNF-α and dexamethasone (Dex) at concentration of 1.0×10-6 mol/L, or TNF-α with Dex (at concentration of 1.0×10-6mol/L) and SIN at different concentrations (0,25, 0.5, and 1.0 mmol/L) (co-treated groups). VCAM-1 expression was detected by flow cytometry (FCM). Results SIN inhibited expression of VCAM-1 in TNF-α-induced HUVECs, the best effect was shown in the 1.0 mmol/L SIN treated group. VCAM-1 decreased more markedly in the co-treated groups. Conclusion SIN inhibits TNF-α-induced VCAM-1 expression on HUVECs in vitro, and SIN maybe synergistic with Dex in inhibiting TNF-α-induced VCAM-1 expression on HUVECs in vitro.