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随机扩增多态性DNA分子标记研究进展及在中药鉴定中的应用
引用本文:刘石泉,周根余,李小军,路群,余庆波. 随机扩增多态性DNA分子标记研究进展及在中药鉴定中的应用[J]. 时珍国医国药, 2004, 15(7): 431-434
作者姓名:刘石泉  周根余  李小军  路群  余庆波
作者单位:上海师范大学生命与环境科学学院生物系遗传学实验室,200234
基金项目:上海市高等学校科学技术发展基金项目 (No.0 3DZ0 2 )
摘    要:随机扩增多态性DNA是一种兼稳定性和多态检出率高的DNA分子标记技术之一,本文综述了RAPD技术原理、操作过程、优势、不足和应对措施以及该技术的扩展、在中药分类、鉴定等中的应用。

关 键 词:随机扩增多态性DNA RAPD扩展 中药鉴定 应用
文章编号:1008-0805(2004)07-0431-04
修稿时间:2004-01-05

Advances in Molecular Marking of Random Amplified Polymorphic DNA(RAPD) and Its Applications in the Authentication of Traditional Chinese Drug(TCD)
LIU Shi-quan,ZHOU Gen-yu,LI Xiao-jun,LU Qun,YU Qing-bo. Advances in Molecular Marking of Random Amplified Polymorphic DNA(RAPD) and Its Applications in the Authentication of Traditional Chinese Drug(TCD)[J]. Lishizhen Medicine and Materia Medica Research, 2004, 15(7): 431-434
Authors:LIU Shi-quan  ZHOU Gen-yu  LI Xiao-jun  LU Qun  YU Qing-bo
Abstract:Random amplified polymorphic DNA (RAPD), a DNA molecular marking technology, is one of reliable and powerful approaches in detecting polymorphism loci. This paper presents the RAPD's principle, process, superiorities, shortcoming and its remedied measures. Expansibility of RAPD and its applications in the authentication of traditional Chinese drug (TCD) are also studied.
Keywords:Random amplified polymorphic DNA(RAPD)  Expansibility of RAPD  Authentication of traditional Chinese drug(TCD)  Applications
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